RP-HPLC-DAD法对银杏叶胶囊质量监控研究
Study on Quality Control for the Ginkgo Leaf Capsule by RP-HPLC-DAD Method
杨兴变 1李洪庆1
作者信息
- 1. 贵州大学,贵州省生化工程中心,贵州贵阳550025
- 折叠
摘要
[目的]利用色谱指纹图谱技术建立银杏叶胶囊质量监控方法.[方法]采用RP-HPLC-DAD方法和中药色谱指纹图谱相似度软件对银杏叶胶囊进行了质量监控研究.色谱柱为InertsilRODS-3 C18 (4.6 mm ×250 mm,5μm);流动相为0.4％磷酸水(A)-乙腈(B)系统,梯度洗脱;流速为1.00 mL/min:柱温为30℃;检测波长为360 nm.[结果]银杏叶胶囊与对照品全峰匹配,其相似度为0.901 ～0.933.[结论]该方法操作简便、稳定、重复性好,为银杏叶胶囊质量控制提供实践依据及有效方法.
Abstract
[Objective] To establish the quality control method for the ginkgo leaf capsules by chromatographic fingerprint.[Method] RPHPLC-DAD method and the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint were used to analyze the qualities of the ginkgo leaf capsules.A InertsilR ODS-3 C18 (4.6 mm× 250 mm,5 μm) was used as analysis column and 0.4％ phosphoric acid solution (A) / acetonitrile (B) as mobile phase at the flow rate of 1.00 mL/min with gradient elution.The column temperature was 30 ℃ and detecting wavelength was 360 nm.[Result] Compared with the reference substance,the similarities of the ginkgo leaf capsules were from 0.901 to 0.933.[Conclusion] The method is proved convenient,stable and repeatable,the study can provide practical basis and effective method for the quality control of the ginkgo leaf capsule.
关键词
银杏胶囊/质量监控/色谱指纹图谱Key words
Ginkgo leaf capsules/Quality control/Chromatographic fingerprint引用本文复制引用
基金项目
贵州大学博士基金(X060046)
贵州省科技厅重大专项子项目(黔科合重大专项字20086020)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据