酸性水与碱性水中Mg2+浓度对黑眶蟾蜍蝌蚪存活率的影响
The Influence of Magnesium Concentration for the Survival Rate of Bufo melanostictus Tadpoles in the Acidity Water and Alkaline Water
叶志强 1刘文华 1罗斯特 2徐大德 1赵健荣1
作者信息
- 1. 肇庆学院生命科学学院,广东肇庆 526062
- 2. 厦门大学生命科学学院,福建厦门 361102
- 折叠
摘要
[目的]研究野外黑眶蟾蜍(Bufo melanostictus)蝌蚪的生存状况.[方法]研究分别在碱性水(pH 7.5 ～8.0)以及酸性水(pH5.5 ～6.0)2种模拟环境条件下,通过急性毒性试验方法以不同的浓度梯度Mg2+对黑眶蟾蜍蝌蚪进行24和48 h的胁迫试验.[结果]在相同浓度梯度和相同观察时间下,黑眶蟾蜍蝌蚪在碱性水中的存活率低于酸性水;不同pH的水环境与Mg2+的协同作用显著影响黑眶蟾蜍蝌蚪的48 h存活率(F7.32=3.373,P＜0.05);黑眶蟾蜍蝌蚪在酸性水环境下适宜生存的Mg2+浓度范围为1 418.41 ～1 590.54 mg/L,半致死浓度(LC50)为2 357.32 mg/L,安全浓度为521.51 mg/L;在碱性水环境下适宜生存的Mg2浓度范围为1 264.91～1 418.41 mg/L,LC50为1 879.81 mg/L,安全浓度为533.99 mg/L.[结论]该研究可为今后黑眶蟾蜍的保护提供理论依据.
Abstract
[Objective] The aim was to study the survival status of Bufo melanostictus tadpoles in the field.[Method] The regular acute toxicity test was carried out that Bufo melanostictus tadpoles were breed in the alkaline water (pH 7.5-8.0) and acidity water (pH 5.5-6.0)with different Mg2+ concentration gradient for 24 and 48 h.[Result] The results showed that the survival rate of Bufo melanostictus tadpoles in the alkaline water was lower than the acidity water in the same condition.Synergistic effects of different pH and Mg2+ concentration had a significant effect on the survival rate of Bufo melanostictus tadpoles in the 48 h toxicity experiment(F7.32 =3.373,P ＜ 0.05).In the acidity water environment,48 h toxicity experiment results showed that the optimal Mg2+ concentration was 1 418.41-1 590.54 mg/L,the median lethal concentration (LC50) was 2 357.32 mg/L and the safe concentration was 521.51 mg/L.By contract,these three indexes in the alkaline water environment were 1 264.91-1 418.41,1 879.8 1 and 533.99 mg/L,respectively.[Conclusion] The study can provide theoretical basis for protection of Bufo melanostictus.
关键词
黑眶蟾蜍/蝌蚪/存活率/Mg2+/急性毒性试验Key words
Bufo melanostictus/Tadpoles/Survival rate/Mg2+/Acute toxicity test引用本文复制引用
基金项目
国家自然科学基金(31271124)
广东省自然科学基金(s2013010016062)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据