南烛叶多酚氧化酶最适提取条件的研究
Study on Optimum Extraction Conditions of Polyphenol Oxidase from Vaccinium bracteatum Thunb Leaf
冯佳丽 1何新叶 1笪荣辉 1金奎 1何志勇 1陈洁1
作者信息
- 1. 江南大学食品科学与技术国家重点实验室,江苏无锡214122
- 折叠
摘要
[目的]深入探讨南烛叶多酚氧化酶(PPO)的酶学性质,对南烛叶PPO的最适提取条件进行研究.[方法]以南烛叶为原料,分别研究了交联聚乙烯吡咯烷酮(PVPP)添加含量、料液比、浸提时间和pH各因素对其提取的PPO酶活的影响.[结果]试验表明,适量南烛叶粉末,以料液比1:15 g/mL,加入含9％ PVPP(W/V) pH 7.6的柠檬酸-磷酸氢二钠缓冲液(0.1 mol/L),匀浆并于4℃下浸提9h,过滤,滤液经冷冻离心(4℃,12 000 r/min,20 min)后得到酶活最高的PPO酶液(11 335 U/mL).[结论]研究结果可为促进南烛叶的工业化应用提供参考.
Abstract
[Objective] The aim was to explore the enzymatic properties of PPO from Vaccinium bracteatum Thunb leaf and research the optimum extraction conditions of PPO.[Method] Effects of PVPP content,solid-liquid ratio,extraction time,pH on PPO enzyme activity were studied respectively.[Result] The results showed that appropriate amount of V.bracteatum power was added into citric acid-phosphate buffer (0.1 mol/L pH 7.6) containing 9％ PVPP(W/V) with solid-liquid ratio 1:15 g/mL,extracted at 4 ℃ for 9 h,through filtering,centrifuging at 4 ℃,12 000 r/min for 20 minutes,PPO with the highest enzyme activity (11 335 U/mL) was obtained.[Conclusion] The study can provide reference for promoting industrialized application of V.bracteatttm leaf.
关键词
南烛叶/多酚氧化酶/酶活/提取Key words
Vaccinium bracteatum Thunb leaf/Polyphenol oxidase (PPO)/Enzyme activity/Extraction引用本文复制引用
基金项目
大学生创新创业训练计划项目(2015157Y)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据