微波消解-石墨炉原子吸收法测定川芎镉含量方法学研究
Determination of Cadmium Concentration of Chuanxiong Rhizoma by Microwave Digestion-Graphite Furnace Atomic Absorption
李阳 1韩桂琪 1何难 1黄彪 1彭成 1国锦琳1
作者信息
- 1. 成都中医药大学中药材标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137
- 折叠
摘要
[目的]建立准确测定川芎中镉含量的分析方法.[方法]微波消解法消化经粉碎混匀的川芎块根样品0.1g,消解总时间25 min,消化液赶酸后稀释定容100 mL,以石墨炉原子吸收法测定总镉浓度.选择1.0 g/L磷酸作基体改进剂提高灰化温度至550℃.[结果]方法检出限为0.08 mg/kg,线性范围为0.08～2.00 μg/L,日内精密度的RSD为0.2％ ～6.5％.测定5个种植区域的川芎样品共48份的加标样品回收率为96.0％～ 109.6％.[结论]该方法测定川芎中镉含量灵敏、准确、高效、易普及,为准确掌握川芎镉含量分布提供了可靠的定量方法.
Abstract
[Objective] To establish an analysis method to accurately determine the cadmium concentration of Chuanxiong Rhizoma.[Method] The tuberous root of Chuanxiong Rhizoma was pulverized and 0.1 g of the powder was digested by microwave.After 25 min of digestion,the acid was drove by heating and diluted to 100 mL.The cadmium concentration of the sample solution was detected by graphite furnace atomic absorption.1.0 g/L phosphoric acid was used as the matrix modifier and the ashing temperature was raised to 550 ℃.[Result] The detection limit was 0.08 mg/kg;linear range was 0.08-2.0 pμg/L;RSD of intraday precision was 0.2％-6.5％.The recovery rates of 48 samples were 96.0％-109.6％ in five planting areas.[Conclusion] This method is sensitive,effective and accurate to detect the cadmium concentration in Chuanxiong Rhizoma,and provides reliable quantitative method for the investigation of accurate distribution of cadmium concentration in Chuanxiong Rhizoma.
关键词
镉含量/川芎/微波消解/石墨炉原子吸收法/定量测定Key words
Cadmium concentration/Chuangxiong Rhizoma/Microwave digestion/Graphite furnace absorption/Quantitative analysis引用本文复制引用
基金项目
国家自然科学基金(81373920)
国家自然科学基金(30801522)
成都中医药大学科技发展基金(030021067)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据