拟南芥莲座叶芥子油苷组成和含量对缺硫胁迫的响应
Response of Glucosinolate Composition and Content in Arabidopsis thaliana Rosette Leaves to Sulphur Deficiency
钟海秀1
作者信息
- 1. 黑龙江省科学院自然与生态研究所,黑龙江哈尔滨150040;东北林业大学盐碱地生物资源环境研究中心,黑龙江哈尔滨150040
- 折叠
摘要
[目的]探讨缺硫胁迫下拟南芥(Arabidopsis thaliana)生长到12片莲座叶时芥子油苷组成和含量的变化规律.[方法]以模式植物拟南芥为原材料,通过水培方法进行缺硫胁迫处理,采用高效液相色谱-质谱联用(HLPC-MS)法分析芥子油苷的组成和含量.[结果]缺硫胁迫处理48h,拟南芥莲座叶中检测出7种脂肪族芥子油苷、4种吲哚族芥子油苷.缺硫胁迫对吲哚族芥子油苷含量影响不显著,对脂肪族芥子油苷含量影响显著.[结论]试验结果为探索缺硫胁迫下芥子油苷的代谢途径提供了理论依据.
Abstract
[Objective] To discuss the change rule of glucosinolate composition and content under sulphur deficiency in rosette leaves of Arabidopsis thaliana during the vegetative stage.[Method] Taking A.thalianaas as experimental materials,hydroponic method was used for sulfur deficiency treatment.Composition and content of glucosinolate were analyzed by HLPC-MS.[Result] Seven kinds of aliphatic glucosinolates and four kinds of indole glucosinolates were detected in rosette leaves of A.thaliana.The content of indole glucosinolate was not significant correlated with sulphur deficiency,while content of aliphatic glucosinolate was significantly correlated with sulphur deficiency.[Conclusion]This research provides theoretical foundation for the metabolic pathways of glucosinolates under sulphur deficiency.
关键词
缺硫胁迫/拟南芥/芥子油苷Key words
Sulphur deficiency/Arabidopsis thaliana/Glucosinolate引用本文复制引用
基金项目
黑龙江省应用技术研究与开发计划项目(PS13H04)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据