石榴皮有效抑菌部位萃取工艺的比较分析
Comparative Analysis of the Extraction Technology of Effective Antibacterial Ingredients from Pomegranate Rind
张坤 1林茂 1李忠琴 1刘宏伟 1熊静 1赖晓健1
作者信息
- 1. 集美大学水产学院,福建厦门361021;鳗鱼现代产业技术农业部工程研究中心,福建厦门361021
- 折叠
摘要
[目的]优化石榴皮有效抑菌部位的萃取工艺.[方法]采用超声辅助萃取法,选取纯水、甲醇、乙醇、丙酮、乙酸乙酯、正己烷6种溶剂萃取石榴皮抑菌药液.通过琼脂平板法测定石榴皮抑菌药液对9株水产常见致病菌的最低抑菌浓度,正交试验方法考察溶剂浓度、料液比、温度、超声时间对抑菌率的影响,进一步优化石榴皮抑菌药液的萃取工艺.[结果]比较不同溶剂抑菌效果,表明乙醇为最佳萃取溶剂,其萃取部位平均最低抑菌浓度为0.517 mg/mL.在乙醇体积分数70％、料液比1∶20 g/mL、萃取温度60 ℃、超声时间40 min的最佳萃取工艺条件下,萃取药液平均最低抑菌浓度为0.442 mg/mL.[结论]研究可为石榴皮在水产养殖细菌性疾病防控中的应用和石榴皮抗菌剂的研制及工业化生产提供理论依据.
Abstract
[Objective] To optimize the extraction technology of antibacterial ingredients from pomegranate rind.[Method] Six kinds of solvents (water,methanol,ethanol,acetone,ethyl acetate and n-hexane) were used to extract antibacterial ingredients from pomegranate rind.The minimal inhibitory concentrations (MIC) of nine common strains of main aquatic pathogenic bacteria were tested by agar dilution method.The orthogonal experimental was designed to research the effects of solvent concentration,solid-liquid ratio,ultrasonic temperature and ultrasonic time on antibacterial rate.Extraction technology of antibacterial ingredients from pomegranate rind was further optimized.[Result] By comparing with the antibacterial effects of different solvents,ethanol was chosen as the best extraction solvent and the average minimum inhibitory concentration was 0.517 mg/mL.The optimal extraction technology was 70％ ethanol concentration,1∶20 g/mL solid-liquid ratio,60 ℃ extraction temperature and 40 min ultrasonic time.Under this condition,the minimum inhibitory concentration was 0.442 mg/mL.[Conclusion] This research provides theoretical basis for the application of pomegranate rind in bacterial disease prevention and control of aquaculture and for the development and industrialized production of pomegranate rind antibacterial agent.
关键词
石榴皮/超声辅助萃取/抑菌/正交试验Key words
Pomegranate rind/Ultrasonic-assisted extraction/Antibacteria/Orthogonal test引用本文复制引用
基金项目
公益性行业(农业)科研专项项目(201203085)
国家海洋局海洋经济创新发展区域示范专项项目(2012FJ03)
福建省教育厅项目(JA15260)
鳗鲡现代产业技术教育部工程研究中心开放基金(RE201306)
福建省科技厅重点项目(2013Y0089)
厦门市科技局项目(3502Z20133015)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据