复合外源物质对玉米幼苗抗寒性的影响
Effects of Composite Exogenous Substance on Cold Resistance of Maize Seeding
丁昊 1杨晓宇 1李隆 1赵璐 1马秀梅 1张继1
作者信息
- 1. 西北师范大学生命科学学院,甘肃兰州730070
- 折叠
摘要
[目的]明确复合外源物质对玉米幼苗抗寒性的影响以及抗寒机理.[方法]以豫玉22号、金穗1203和宇辉早甜粘玉米品种为材料,利用人工模拟低温方法,研究在5℃低温和不同时长处理下复合外源物质对玉米幼苗抗寒性的影响.[结果]在5℃低温条件下,随着低温胁迫时间的延长,3种玉米品种遭受低温伤害的程度逐渐加剧,尤其在胁迫第5天最明显.在5℃低温条件下,玉米幼苗叶片中丙二醛含量、游离脯氨酸含量、相对电导率和可溶性蛋白含量随着胁迫时间的延长呈增加趋势,恢复生长3d后又开始下降;超氧化物歧化酶活性随着胁迫时间的延长不断降低;可溶性糖含量先上升后下降,在第3天胁迫时达到最大.在5℃低温条件下,随着低温胁迫时间的延长,复合外源物质对玉米幼苗叶片中丙二醛含量、游离脯氨酸含量、相对电导率、可溶性糖含量、可溶性蛋白含量和超氧化物歧化酶活性的影响程度越明显.[结论]复合外源物质能增强玉米幼苗适应低温胁迫的能力.
Abstract
[Objective] To research the effects of composite exogenous substance on cold resistance of maize seeding and their mechanism ofcold resistance.[Method] Yuyu 22,Jinsui 1203 and Yuhui early sweet sticky were selected as test materials in this study.Effects of composite exogenous substance on cold resistance of maize seeding was studied at 5 ℃ with different time durations.[Result] At low temperature of 5 ℃,as the three kinds of maize suffered from low temperature for long hours,the injury degenerated especially at the 5th day.At low temperature of 5 ℃,the content of MDA and free proline content as well as relative conductivity and soluble protein content increased as time prolonged in maize seedling leaves.These indexes began to decline after the growth recovery for three days.SOD activity decreased as time prolonged at 5 ℃.However,the content of soluble sugar increased at the beginning and decreased subsequently after reaching the peak on the 3th day.At low temperature of 5 ℃,as the cold stress time prolonged,effects of composite exogenous substance on the content of MDA,free proline,soluble sugar,soluble protein became more obvious,as well as the relative conductivity and activity of SOD in maize seedling leaves.[Conclusion] Composite exogenous substance enhances the adaptability of maize seedlings under low temperature stress.
关键词
复合外源物质/玉米幼苗/低温胁迫/抗寒性Key words
Composite exogenous substance/Maize seedlings/Low temperature stress/Cold resistance引用本文复制引用
基金项目
国家科技支撑计划项目(2012BAD20B06)
国家自然科学基金(31200255)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据