利用psbA-trnH片段鉴定罗汉果和山药破壁草本
Identification of Ultra-fine Granular Powers of SIRAITIAE FRUCTUS and DIOSCOREAE RHIZOMA by psbA-trnH Fragment
郑夏生 1赖智填 2成金乐2
作者信息
- 1. 国家中医药管理局中药破壁草本技术与应用重点研究室,中山市中智药业集团有限公司,广东中山528437;中国中医科学院中药研究所,北京100700
- 2. 国家中医药管理局中药破壁草本技术与应用重点研究室,中山市中智药业集团有限公司,广东中山528437
- 折叠
摘要
[目的]解决罗汉果和山药破璧草本DNA提取困难,而无法用分子生物学鉴定的问题.[方法]通过比较常用DNA提取试剂盒法和改良的CTAB法,利用sbA-trnH片段鉴定罗汉果和山药破壁草本.[结果]改良的CTAB法可从罗汉果和山药的药材、破壁粉末和破壁草本3种形态中成功地提取出基因组DNA,并可进一步利用DNA条形码的psbA-trnH片段进行分子鉴定.[结论]该方法稳定可行,可用于罗汉果和山药的药材、破壁粉末和破壁草本的DNA条形码鉴别.
Abstract
[Objective] To solve the problems of difficult extraction of DNA extraction from ultra-fine granular powers of SIRAITIAE FRUCTUS and DIOSCOREAE RHIZOMA.[Method] Ultra-fine granular powers of SIRAITIAE FRUCTUS and DIOSCOREAE RHIZOMA were identified by psbA-trnH fragment by comparing the traditional DNA extraction kit and improved CTAB method.[Result] The improved CTAB method could successfully extract the medicinal materials,ultra-fine powders and ultra-fine herbs of SIRAITIAE FRUCTUS and DIOSCOREAE RHIZOMA.psbA-trnH fragment in DNA barcode was used for molecular identification.[Conclusion] This method is stable and feasible,and can be used for the DNA barcode identification of medicinal materials,ultra-fine powders and ultra-fine herbs of SIRAITIAE FRUCTUS and DIOSCOREAE RHIZOMA.
关键词
罗汉果/山药/破壁草本/DNA条形码Key words
SIRAITIAE FRUCTUS/DIOSCOREAE RHIZOMA/Ultra-fine granular power of Chinese Herbal Medicine/DNA barcode引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据