快速检测联苯菊酯的胶体金层析试纸条研制
Development of Colloidal Gold Immunochromatograhic Strip for Rapid Detection of Bifenthrin
朱海 1许稳健 1杨星星 1付辉 1李细清 1李远兵 1严义勇 1毕思远 2汪凤林1
作者信息
- 1. 深圳市易瑞生物技术有限公司,广东深圳518101
- 2. 深圳市博英菲生物科技有限公司,广东深圳518102
- 折叠
摘要
[目的]采用免疫胶体金技术,建立一种快速检测联苯菊酯的试纸条方法.[方法]利用有机合成方法制备了联苯菊酯半抗原,并与载体蛋白偶联得到免疫抗原,利用人工免疫的方法获得其单克隆抗体,通过酶联免疫法对制备的单克隆抗体的性能进行了验证.联苯菊酯胶体金层析试纸条是利用单克隆抗体标记胶体金,并将联苯菊酯抗原与羊抗鼠IgG分别喷制到硝酸纤维膜上,与样品垫、吸水垫组装在PVC衬板上制备而成.[结果]联苯菊酯胶体金层析试纸条检测限为500～ 800 μg/kg,具有速度快、简单等优点.此外,该试纸条与大部分的拟除虫菊酯没有明显的交叉反应,利用该试纸条可实现对苋菜中联苯菊酯添加试验的准确判定.[结论]胶体金试纸条方法具有前处理简单、检测速度快、可以肉眼判断结果等优点,该试纸条有望实现对果蔬中联苯菊酯残留的快速筛查.
Abstract
[Objective] A rapid strip detection method for bifenthrinis was established based on immune colloidal gold technology.[Method] The hapten of bifenthrin was prepared via organic synthesis method,and was conjugated with the carrier protein to obtain the antigen.Monoclonal antibody was obtained with the artificial immune method and its affinity was verified with the enzyme immunoassay.The colloidal gold immunochromatographic strip was prepared with monoclonal antibody conjugated gold colloid which is stored in microwells,and a PVC liner which is assembled with nitrocellulose membrane sprayed with bifenthrin antigen and goat anti-mouse IgG,sample pad and absorbent pad.[Result] The strip had a detection limit in the range of 500-800 μg/kg with the advantages of fastness,easiness,etc.In addition,the strip did not cross-react with most of the pyrethroid pesticides.With the strip,the detection of bifenthrin in edible amaranth was accurately reported.[Conclusion] The colloidal gold strip method has advantages of simple pre-treatment,rapid detection and has potential of fast screening bifenthrin residues in fruits and vegetables.
关键词
联苯菊酯/单克隆抗体/胶体金/快速检测/免疫层析Key words
Bifenthrin/Monoclonal antibody/Colloidal gold/Rapid detection/Immunochromatography引用本文复制引用
基金项目
广东省科学技术厅项目(GCZX20140509163151273)
深圳市科技创新委员会项目(2013B040402001)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据