欧美杨PdSIKI基因的克隆与功能分析
Cloning and Functional Analysis of PdSIKI from Populus deltoides × Populus nigra
杨李娇 1郭鹏 1许涵杰1
作者信息
摘要
[目的]筛选抗旱节水欧美杨品种.[方法]以欧美杨为材料,通过RT-PCR方法从欧美杨叶片中克隆到抗旱PdSIKI基因,将其转入拟南芥中,研究转PdSIKI基因拟南芥的表型性状和生理指标.[结果]PdSIKI基因开放阅读框为2442 bp,编码813个氨基酸.蛋白质分析表明PdSIKI蛋白含有典型的类受体蛋白激酶高度保守的丝氨酸/苏氨酸结构域和跨膜区域.荧光定量PCR分析表明该基因在顶端叶中表达量最高,功能叶、根和茎中则无表达.逆境胁迫分析表明该基因受NaCl、ABA和干旱诱导表达明显.与野生型相比,干旱处理下转PdSIKI基因拟南芥表现出更强的生长状态,且叶绿素含量降低,丙二醛含量升高.[结论]超表达PdSIKI基因提高了拟南芥抗干旱能力.
Abstract
[Objective] The aim was to screen out drought resistance and water-saving Populus deltoides × Populus nigra varieties.[Method] With Populus deltoides × Populus nigra as materials,drought resistance PdSIKI gene was cloned from Populus deltoides × Populus nigra leaves with RT-PCR method then transferred into Arabidopsis thaliana,the phenotypic traits and physiological indicators were studied.[Result] The cDNA of PdSIKI was 2 442 bp and contained a single open reading frame of 813 amino acid residues.Protein sequence analysis showed the PdSIKI has a serine/threonine protein kinase domain and transmembrane.Real time-PCR indicated that the mRNA accumulation of PdSIKI was strongly expressed in immature leaves,but not in functional leaves,roots and stem and induced by salt stress,ABA and drought.Additionally,compared with the control,transgenic PdSIKI arabidopsis performed stronger growth status.Chlorophyll content decreased and MDA content increased in drought stress.[Conclusion] PdSIKI gene can improve drought resistance ability of Arabidopsis thaliana.
关键词
欧美杨/PdSIKI基因/克隆/表达/干旱Key words
Populus deltoides × Populus nigra/PdSIKI/Cloning/Expression/Drought stress引用本文复制引用
基金项目
科技部火炬计划项目(2012GH531899)
科技部星火项目(2013GA651006)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据