一种纤维素酶产生菌的筛选方法
A Screening Method for Cellulose-producing Strains
徐艳 1刘玉玲 2严汉彬 3伍德政3
作者信息
- 1. 河源职业技术学院机电工程学院,广东河源517000;汕头大学理学院,广东汕头515063
- 2. 河源市环境保护技术中心,广东河源517000
- 3. 河源职业技术学院机电工程学院,广东河源517000
- 折叠
摘要
[目的]获得一种简单高效的纤维素酶产生菌的筛选方法.[方法]基于扩散原理,采用滤纸条扩散试验筛选纤维素酶产生茵.[结果]获得2株纤维素酶产生茵菌株X1和X2.经DNS法检验,菌株X1的FPA酶和CMC酶活力分别为12.3和8.3U,菌株X2的FPA酶和CMC酶活力分别为8.8和7.8U.[结论]滤纸条扩散试验可作为一种便捷高效的筛选高质量纤维素酶产生茵的方法.
Abstract
[Objective] To obtain a screening method for cellulose-producing strains,which was simple and effective.[Method] Based on the diffusion principle,filter paper diffusion experiment was used to screen the cellulose-producing strains.[Result] Two cellulose-producing strains (X1,X2) were obtained.DNS test showed that the activities of FPAase and CMCase of two strains were 12.3,8.3 ;7.8,8.8 U,respectively.[Conclusion] Results indicated that filter paper diffusion experiment could be used as a new efficient method to screen celluloseproducing strains.
关键词
扩散/纤维素酶/筛选Key words
Diffusion/Cellulose-producing strains/Screening引用本文复制引用
基金项目
广东省科技项目(2015A040404014)
河源市科技项目(2013-139)
河源市科技项目(源科[2014]12号)
出版年
2016