南重楼中4种重楼皂苷超声提取工艺
Ultrasonic Extraction Technology of Four Kinds of Polyphyllin in Paris vietnamensis
刘江 1段志刚 1段宝忠 1夏从龙1
作者信息
- 1. 大理大学药学与化学学院,云南大理671000
- 折叠
摘要
[目的]优化南重楼中4种重楼皂苷提取的最佳工艺.[方法]采用L9(34)正交试验,对提取溶剂的单次料液比、单次提取时间、初始提取温度、提取次数4因素进行优化考察,HPLC法测定4种重楼皂苷含量并作为考察指标.[结果]最佳提取工艺单次料液比为15倍的甲醇,每次提取20 min,初始提取温度为40℃,提取次数为3次.[结论]该提取工艺操作简单、合理、可行、重复性好、稳定可靠,可用于南重楼中4种重楼皂苷的提取.
Abstract
[Objective] To select the optimal extraction procedure of polyphyllin from four kinds of Paris vietnarnensis.[Method] L9 (34) orthogonul test was adopted to investigate the four factors of solid-liquid ratio,extraction time,initial extraction temperature and extraction times.Polyphyllin contents in four kinds of P.vietnamensis were detected by HPLC method.[Result] The optimal extraction condition was as follows:15 times of methanol,20 min extraction time,40 ℃ extraction temperature,and three extraction times.[Conclusion] This extraction technology is simple,stable,reliable,reasonable and feasible,and can be used for the extraction of polyphyllin from four kinds of P.vietnamensis.
关键词
南重楼/重楼皂苷/HPLC法/提取工艺/正交试验Key words
Paris vietnamensis/Polyphyllin/HPLC method/Extraction technology/Orthogonal design引用本文复制引用
基金项目
云南省科技厅面上项目(2013FB059)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据