诱导大豆抗胞囊线虫病和根腐病的生防细菌研究
Study on the Biocontrol Bacteria Inducing Soybean Cyst Nematode and Soybean Root Rot Diseases
闫继辰 1王媛媛 1朱晓峰 1刘晓宇 1陈立杰 1段玉玺1
作者信息
- 1. 沈阳农业大学植物保护学院植物线虫学实验室,辽宁沈阳110161
- 折叠
摘要
[目的]筛选诱导大豆抗胞囊线虫病和根腐病的生防细菌,挖掘高效的新型微生物代谢物种衣剂.[方法]从诱导抗性角度,以大豆胞囊线虫和大豆根腐病菌为靶标,通过对2 400株细菌发酵液包衣大豆种子处理、大田和温室盆栽试验,筛选诱导大豆抗胞囊线虫病和根腐病的生防细菌.[结果]获得Sneb152、Sneh572、Sneb877、Sneb1076、Sneb1401和Sneb1499共6株对病原物具有诱抗活性的生防细菌,6株生防细菌均可诱导大豆抗胞囊线虫病,抑制率超过74.98％,且能显著促进大豆生长,增产效果明显;菌株Sneb572和Sneb1076对大豆根腐病也有一定防效.[结论]优良诱抗生防菌株Sneb572和Sneb1076用于处理大豆种子前景广阔.
Abstract
[Objective] To screen the biocontrol bacterium inducing soybean cyst nematode and soybean root rot diseases,and to find high-efficient new microbial metabolites seed coatings.[Method] From the aspect of induced resistance,soybean cyst nematode and soybean root rot pathogen were used as the targets.A total of 2 400 coating soybean seeds were processed in bacteria fermentation liquid.[Result] After field and greenhouse screening,6 strains of biocontrol bacteria (Sneb152,Sneb572,Sneb877,Sneb1076,Sneb1499 and Sneb1401) were obtained,which had resistance activity to the pathogen.The 6 strains of biocontrol bacteria could all induce soybean cyst nematode with the inhibition rate being more than 74.98％.They could significantly promote the growth of soybean and had obvious yield increasing effect.Besides,strains Sneb572 and Sneb1076 also had certain control effects on soybean root rot.[Conclusion] Sneb572 and Sneb1076 have broad application prospects in soybean seed treatment.
关键词
生防菌/大豆胞囊线虫病/根腐病/促生长/诱导抗性Key words
Biocontrol bacteria/Soybean cyst nematode/Root rot disease/Growth promotion/Induced resistance引用本文复制引用
基金项目
行业公益性项目(201503114-12)
国家自然科学基金重点项目(31330063)
国家自然科学基金面上项目(31171569)
现代农业产业技术体系建设专项(CARS-04-PS13)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据