四妙丸中盐酸小檗碱和苍术素的含量测定方法研究
Content Determination of Berberine Hydrochloride and Atractylodin in Simiao Pills
王艳秋 1刘向前1
作者信息
- 1. 沈阳市中医药学校,辽宁沈阳110300
- 折叠
摘要
[目的]建立四妙丸中盐酸小檗碱和苍术素的含量测定方法.[方法]采用高效液相色谱法,色谱柱为Promosil C18色谱柱(250 mm ×4.6mm,5μm);流动相为乙腈(A)～0.1％H3PO4溶液(B),梯度洗脱,洗脱程序为:0～5 min,25％A;5～7 min,25％～90％ A;7～15 min,90％A;检测波长340 nm.[结果]盐酸小檗碱和苍术素的线性范围分别为0.25～2.50和0.32～3.20 μg,平均回收率分别为97.5％和96.8％,RSD分别为2.6％和2.8％.[结论]该方法准确、可靠,可作为四妙丸的质量控制方法.
Abstract
[Objective] To establish a method for the content determination of berberine hydrochloride and atractylodin in Simiao pills.[Method] The HPLC method was adopted with Promosil C18 chromatography column(250 mm× 4.6 mm,5 μm).The mobile phase was acetonitrile (A)-0.1％ H3PO4 solution (B) with gradient eluent,the elution program was 0-5 min,25％ A;5-7 min,25％-90％ A;7-15 min,90％ A.The detection wavelength was 340 nm.[Result] The linear ranges of berberine hydrochloride and atractylodin were 0.25-2.5 and 0.32-3.2 μg,respectively.The average recovery rates of the two components in Simiao pills were 97.5％ and 96.8％,while RSD were 2.6％ and 2.8％,respectively.[Conclusion] The method is accurate and reliable for the quality control of Simiao pills.
关键词
四妙丸/盐酸小檗碱/苍术素/含量测定Key words
Simiao pill/Berberine hydrochloride/Atractylodin/Content determination引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据