獭兔酪氨酸酶相关蛋白1(Tyrp1)基因序列分析及外显子多态性研究
Analysis of Polymorphism of Exons in Tyrosinase-Related Protein 1 (Tyrp1) Gene in Rex Rabbit(Oryctolagus cuniculus)
郝晔 1施丽娟 1闫晓荣 1赵博昊 1朱杰 1陈阳 1翁巧琴 2吴信生1
作者信息
- 1. 扬州大学动物科学与技术学院,江苏扬州225009;江苏省动物繁育与分子设计重点实验室,江苏扬州225009
- 2. 浙江省余姚市欣农兔业有限公司,浙江余姚315400
- 折叠
摘要
[目的]探索獭兔酪氨酸相关蛋白1(Tyrp1)基因在毛色形成过程中的分子机制.[方法]对獭兔Tyrp1基因序列的核苷酸序列、编码产物的基本理化性质等进行预测和分析,同时利用PCR-SSCP(Single strand conformation polymorphism analysis of polymerase chain reaction products)技术检测Tyrp1基因CDS序列的多态性分布状况.[结果]信息学分析发现,TYRP1蛋白是不稳定的亲水性蛋白,有信号肽,具有酪氨酸酶家族特征性功能结构域.多态性检测发现仅在Tyrp1基因CDS的483 bp处存在1个C→T的同义突变,位于外显子2上,该碱基的突变没有造成氨基酸的改变(AUC→AUT,异亮氨酸).Hardy-Weinberg平衡检验表明,青紫蓝色獭兔群体处于非平衡状态,白色獭兔群体处于平衡状态.青紫蓝兔群体为中度多态信息含量(0.25＜PIC＜0.50),白色獭兔群体为低度多态信息含量(PIC＜0.25).[结论]Tyrp1基因对獭兔的毛色有一定的影响.该研究结果可为獭兔毛色的选种选育提供一定的参考.
Abstract
[Objective] To discuss the molecular mechanism of tyrosinase-related protein 1 (Tyrp1) gene of Rex rabbit (Oryctolagus cuniculus) during the formation of hair color.[Method] The Tyrp1 gene sequence,the nucleotide sequence,and the basic physical and chemical properties of coding products were forecasted and analyzed.At the same time,PCR-SSCP (single-strand conformational polymorphism) was used to detect the distribution of CDS sequences of Tyrp1.[Result] Bioinformatics analysis found out that Tyrp1 protein was an unstable hydrophilic protein,which had a signal peptide and a functional domain of the tyrosinase family.The polymorphism detection result indicated that the exon 2 of Tyrp1 in 29 bp (the gene CDS 483 bp) had a C→T mutation,and the nucleotide mutation did not lead to the amino acid change (AUC→AUT,isoleucine).Hardy-Weinberg equilibrium test showed that the Chinchilla Rex rabbit population was at Hardy-Weinberg disequilibrium,the white Rex rabbit population was at Hardy-Weinberg equilibrium.PIC of chinchilla Rex rabbit population was of medium polymorphism (0.25 ＜ PIC ＜ 0.50),white Rex rabbit population was of low polymorphism (PIC ＜ 0.25).[Conclusion] Tyrp gene has certain effects on the hair color of Rex rabbit.This research provides certain references for the selection of hair color of Rex rabbit.
关键词
獭兔/Tyrp1/SNPs/序列分析Key words
Rex rabbit/Tyrp1/SNPs/Sequence analysis引用本文复制引用
基金项目
现代农业产业技术体系建设专项(CARS-44-1)
江苏高校优势学科建设工程资助项目(PAPD 2014-134)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据