黔产吊石苣苔中总黄酮提取工艺优化
Extraction Process Optimization of Total Flavonoids from Lysionotus pauciflorus Grown in Guizhou Province
汪志勇 1刘红 1徐红 1江峰 2朱念南1
作者信息
- 1. 贵州医科大学基础医学院,贵州贵阳550004
- 2. 贵州医科大学公共卫生学院,贵州贵阳550004
- 折叠
摘要
[目的]探索黔产吊石苣苔总黄酮的最佳提取工艺条件.[方法]以吊石苣苔总黄酮的提取率为考察指标,以乙醇为提取剂,采用单因素和正交试验考察了乙醇浓度、料液比、浸提次数、提取时间对吊石苣苔总黄酮提取率的影响.[结果]吊石苣苔总黄酮的最适宜提取工艺条件是乙醇浓度55％、料液比1∶20、提取时间2h、浸提2次,在该条件下黔产吊石苣苔总黄酮提取率为5.152％.[结论]该工艺可为吊石苣苔的进一步综合开发提供参考.
Abstract
[Objective] To explore the optimal extraction conditions of total flavonoids from L.pauciflorus.[Method] Extraction rate of total flavonoids from L.pauciflorus was used as the investigation index,single factor test and orthogonal test were carried out by using ethanol as the extraction solvent,so as to research the effects of ethanol concentration,solid-liquid ratio,extraction time and extraction times on the extraction rate of total flavonoids.[Result] The optimal extraction conditions of total flavonoids from L.pauciflorus were as follows:55％ ethanol concentration,1 ∶ 20 solid-liquid ratio,3 h extraction time and 2 extraction times.Under these conditions,extraction rate of total flavonoids could reach 5.152％.[Conclusion] This technology provides references for the further comprehensive development of L.pauciflorus.
关键词
吊石苣苔/总黄酮/提取工艺/优化Key words
Lysionotus pauciflorus/Total flavonoids/Extraction technology/Optimization引用本文复制引用
基金项目
贵州省科技厅联合基金(黔科合LH字20147080)
贵州省教育厅自然科学研究青年项目(黔教科研发2013405号)
2015年国家级大学生创新创业训练项目(201510660026)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据