橡胶草高效再生体系的建立和优化
Establishment and Optimization of High Efficient Regeneration System of Taraxacum kok-saghyz Rodin
赵李婧 1李永梅 1曹新文 1马海霞 1闫洁 1祝建波1
作者信息
- 1. 石河子大学生命科学学院,新疆石河子832000
- 折叠
摘要
[目的]优化橡胶草再生和遗传转化体系.[方法]以橡胶草茎和叶为外植体,以1/2MS和MS为基本培养基,研究不同激素浓度配比、外植体类型和激素浓度对橡胶草不定芽诱导和生根的影响.[结果]橡胶草进行组织培养的最佳外植体类型为茎,诱导不定芽形成的最佳激素浓度配比为1.O mg/L 6-BA和0.10 mg/L NAA.诱导不定芽生根的最佳激素浓度为0.10 mg/L NAA,生根率达100％,移栽成活率达95％以上.[结论]建立了橡胶草高效再生体系,对提高橡胶草组织培养的效率和转化率具有重要意义.
Abstract
[Objective] The aim was to optimize Tarquin kok-saghyz Rodin regeneration and genetic transformation system.[Method] With stems and leaves of T.kok-saghyz as explants,1/2MS and MS as the basic medium,effects of different hormone concentrations and explant types on adventitious bud induction and rooting were studied.[Result] The results showed that the best explant for tissue culture was stem,the best hormone concentration ratio of adventitious bud induction was 1.0 mg/L 6-BA and 0.10 mg/L NAA,and the adventitions bud was healthy.The most suitable hormone concentration for adventitious bud rooting was 0.10 mg/L NAA with 100％ rooting rate and 95％ survival rate.[Conclusion] The established high efficient regeneration system of T.kok-saghyz has significance on improving tissue culture efficiency and transformation ratio.
关键词
橡胶草/组织培养/优化Key words
Taraxacum kok-saghyz Rodin/Tissue culture/Optimization引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据