响应面法优化酶法提取油茶籽饼粕多糖工艺
Optimization of Extraction of Polysaccharides from Camellia Seed Cake by Neutral Protease Using Response Surface Methodology
金日生 1徐冰彦 2袁传勋2
作者信息
- 1. 合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230009;中国林业科学研究院林产化学工业研究所,江苏南京210042
- 2. 合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230009
- 折叠
摘要
[目的]提高油茶籽饼粕多糖的提取率.[方法]以榨油后的油茶籽饼粕为原料,利用酶水解与传统热水浸提相结合的方法提取油茶籽饼粕多糖.考察了中性蛋白酶、纤维素酶、半纤维素酶及果胶酶对油茶籽饼粕多糖提取率的影响,对提取工艺中酶解温度、酶解时间、酶添加量分别进行单因素试验,在此基础上利用3因素3水平的响应面法优化工艺参数.[结果]中性蛋白酶法提取油茶籽饼粕多糖的酶解最佳工艺条件为酶解温度40℃、酶解时间120 min、加酶量2.0％,此工艺条件下粗多糖得率为15.66％,是优化前得率7.65％的2.05倍.[结论]该方法提取率高,是一种有效的油茶籽饼粕多糖提取方法.
Abstract
[Objective] To improve the extraction rate of the polysaccharides from camellia seed cake.[Method] With camellia seed cake after oil manufacture as the research material,polysaccharides were extracted by pectinase hydrolysis combined with traditional hot water extraction.Effects of neutral protease,cellulase,hemicellulase and pectinas on the polysaccharide extraction rate were researched.Single factor test was carried out to research the effects of three factors (enzymolysis temperature,enzymolysis time and enzyme dosage) on the polysaccharides extraction rate.Response surface methodology was applied to optimize the optimal conditions.[Result] The optimal extraction technology was as follows:40 ℃ enzymolysis temperature,120 min enzymolysis time and 2.0％ enzyme dosage.Under these conditions,the average extraction rate of polysaccharides was 15.66％,which was 2.05 times before optimization (7.65％).[Conclusion] This method has high extraction rate of polysaccaride,and is a kind of effective method for polysaccharide extraction.
关键词
油茶籽饼粕/多糖/酶法/单因素试验/响应面分析Key words
Camellia seed cake/Polysaccharides/Enzyme method/Single factor test/Response surface methodology引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据