草鱼出血病分子流行病学及GCRV多样性研究
Molecular Epidemiology of Hemorrhage Disease of Grass Carp and the Diversity Research of GCRV
黄毅昌 1雷燕 2杨玉滔 1闫秀英 1简纪常 1吴灶和1
作者信息
- 1. 广东海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点实验室/水产经济动物病害控制广东普通高校重点实验室,广东湛江524088
- 2. 广州利洋水产科技股份有限公司,广东广州510515
- 折叠
摘要
[目的]分析不同基因型GCRV分离株与患草鱼出血病草鱼症状的相关性.[方法]采用RT-PCR检测患病草鱼,应用生物信息学方法分析基因型内和基因型间不同分离株间的差异.[结果]对近3年来草鱼出血病分子流行病学分析发现,患草鱼出血病病鱼多为“肠炎型”症状,且检测发现其病原多属于GCRV基因型Ⅲ型.对GCRV的多样性分析表明,不同分离株同源蛋白的同源率高达93％以上,却被给予完全不同的名称,给GCRV多样性研究带来一定的困难;同一基因型不同分离株同源蛋白间的变异位点较少,且功能位点发生变异的更少;不同基因型不同分离株同源蛋白间的保守位点较少,且这些保守位点中只有极少数位点为功能位点;不同基因型分离株同源结构蛋白间存在较大的差异,且同源非结构蛋白间的差异更为显著.[结论]草鱼出血病不同临床症状可能与其病原基因型不同有关.
Abstract
[Objective] The aim was to analyze the correlation between different genotypes GCRV isolates and symptoms of grass carp with hemorrhage disease.[Method] Using RT-PCR detection,the bioinformatics method was adopted to analyze differences of isolates within and between various genotypes.[Result] The molecular epidemiology of hemorrhage disease of grass carp in recent three years was analyzed.It was found that the symptom of grass carp with hemorrhage disease was "the enteritis type",with the GCRV mostly attributed to genotype Ⅲ.It was indicated that the homologous rates of homologous proteins in diferrent isolates were more than 93％,but those proteins were named with the completely different names.It (highly homologous proteins with different names) brought some difficulties to the research of GCRV diversity.Variable sites were few among homologous proteins in different isolates attributed to the same genotype,and variable function sites were fewer in these variable sites.Conserved sites among homologous proteins in different isolates attributed to different genotypes were few,and the fewer sites among them were functional sites.There were great differences among homologously structural proteins in different isolates attributed to different genotype,and the variation of homologous non-structural proteins was more significant.[Conclusion] It is suggested that different clinical symptoms of hemorrhage disease of grass carp could be related to different pathogen genotypes.
关键词
草鱼呼肠孤病毒/草鱼出血病/分子流行病学/多样性Key words
Grass carp reovirus (GCRV)/Hemorrhage disease of grass carp/Molecular epidemiology/Diversity引用本文复制引用
基金项目
国家重点基础研究发展计划项目(2009CB118704)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据