乙醇-硫酸铵双水相提取马齿苋总黄酮工艺研究
Extraction of Total Flavonoids from Purslane Using Ethanol-Ammonium Sulfate Aqueous Two Phase System
郝红英 1牛美兰 1王研 1樊亚蕾 1孙莉红1
作者信息
- 1. 黄河科技学院医学院,河南郑州450063
- 折叠
摘要
[目的]优化乙醇-硫酸铵双水相提取马齿苋总黄酮工艺.[方法]采用乙醇-硫酸铵双水相体系提取马齿苋中主要成分黄酮,以总黄酮得率为指标,采用单因素和正交试验,考察提取溶剂浓度、料液比、提取时间、提取温度对总黄酮提取工艺的影响,确定乙醇-硫酸铵双水相体系提取马齿苋总黄酮的工艺.[结果]试验最佳提取条件为脱脂马齿苋粉末2.0g,乙醇用量27.50％(W)、硫酸铵16.00％ (W)、料液比1∶20、温度60℃、时间40 min,在此条件下,马齿苋总黄酮提取率为7.23％.[结论]乙醇-硫酸铵双水相提取法提取效率高、操作简便,提取剂价格便宜,是一种很有发展前途的提取方法.
Abstract
[Objective] The aim was to optimize the extraction technology of total flavonoids from purslane using ethanol / ammonium sulfate aqueous two phase system.[Method] The flavonoids trom purslane was extracted by using ethanol / ammonium sulfate aqueous two phase system.With total flavonoids yield as indicator,adopting single factor and orthogonal test,effects of solvent concentration,sloid-liquid ratio,tine,temperature on extraction process were investigated.[Result] The optimal extraction conditions were:2.0 g non-fat purslane powder,ethanol 27.50％ (W),ammonium sulfate 16.00％ (W),solid-liquid ratio 1∶20,temperature 60 ℃,and time was 40 min.Under the above conditions,the extraction rate of total flavonoids was 7.23％.[Conclusion] The extraction efficiency of ethanol-ammonium sulfate aqueous two phase system is high,the operation is simple,and the extracting solvent is cheap,so it is a promisiug extraction method.
关键词
乙醇-硫酸铵双水相/马齿苋/总黄酮/提取工艺/正交设计Key words
Ethanol-ammonium aqueous two phase system/Purslane/Total flavonoids/Extraction technology/Orthogonal design引用本文复制引用
基金项目
郑州市科技局科研项目(20140787)
黄河科技学院大学生实践创新项目(2015XSCXCY008)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据