西藏羊八井高温地热田中度嗜热放线菌多样性及生物活性
Biological Activity and Diversity of Moderately Thermophilic Actinomyccetes from Geothermal Field in the Yangbajing of Tibet
尹明远 1张格杰 1何建清 1燕飞2
作者信息
- 1. 西藏大学农牧学院,西藏林芝 860000
- 2. 浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021
- 折叠
摘要
[目的]探明羊八井地热田放线菌的数量、种类组成、拮抗活性和酶活性.[方法]采用稀释平板法对羊八井地热田底泥和水样放线菌进行分离,通过形态特征、培养特征和16S rRNA基因系统发育分析的方法研究样品中度嗜热放线菌的多样性,并对分离到的放线菌进行抑菌、酶活性及温度耐受试验.[结果]从羊八井温泉底泥和水样中分离到21株中度嗜熟放线菌,分布于放线菌门放线菌纲3个亚目3个科3个属,即链霉菌属(Streptomyces)、拟诺卡氏菌属(Nocardiopsis)和小单孢菌属(Micromonospora).21株中度嗜热放线菌中,14.2％的菌株具有纤维素酶活性,52.3％的菌株具有过氧化氢酶活性,28.6％的菌株具有硝酸盐还原活性,33.3％的菌株可产生硫化氢,只有1株菌对大肠杆菌(E.coil)有抑菌活性,21株中度嗜热放线菌最适生长温度为45 ～55℃,超过65℃都不能生长.[结论]羊八井地热田放线菌多样性较丰富,产酶特性良好,为开发利用地热环境放线菌资源奠定了基础.
Abstract
[Objective] The aim was to explore the quantity,composition,antagonistic activity and enzyme activity of actinomyccetes from geothermal field in Yangbajing.[Method] Thermophilic actinomycetes were isolated via plate dilution from the sediment and water of Yangbajing geothermal field in Tibet Province.Based on the morphology,cultural characteristics and 16S rRNA sequence,diversity of thermophilic actinomycetes were studied,antibacterial activity,enzyme activity and temperature tolerance tests were conducted on isolates.[Result] The results showed that 21 isolates were identified and found to distribute into 3 genus:Streptomyce,Micromonospora and Nocardiopsis.Among those,14.2％ produced cellulase,52.3％ produced catalase,33.3％ produced H2S and 28.6％ could reduce nitatte,and 1 strain had antibacterial activity against Escherichia coil.The optimal growth temperature range for strains was 45-55 ℃ and temperature over 65 ℃ totally blocked the growth.[Conclusion] The discovery of diverse thermophilic actinobacteria from Yangbajing geothermal field indicates a potential source for enzymes.This study lays a foundation for the exploration and utilization of thermophilic actinobacteria resource.
关键词
嗜热放线菌/16S/rRNA/生物活性/多样性Key words
Thermophilic actinomycetes/16S rRNA/Biological activity/Diversity引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据