菌株0206产PHA的发酵条件优化
Optimization of Fermentation Conditions of PHA-producing Strain 0206
朱九滨 1李爽芹 1梁宝东 1李湘利1
作者信息
- 1. 济宁学院生命科学与工程系,山东曲阜 273155
- 折叠
摘要
[目的]优化菌株0206产PHA株的发酵条件.[方法]以菌株0206为试验菌株,对碳源、氮源、培养基pH和培养时间进行了单因素试验和正交试验.[结果]从活性污泥中分离筛选到高产PHA的菌株0206,通过生理生化鉴定,菌株0206为芽孢杆菌属,菌株0206产PHA最优发酵条件为葡萄糖10 g/L,硫酸铵10 g/L,氯化钠2g/L,培养基pH 7.0,培养时间48 h.[结论]优化了菌株0206产PHA的发酵条件,为PHA的发酵提供参考.
Abstract
[Objective] The aim was to study fermentation conditions of PHA-produeing strain.[Method] With strain 0206 as test material,fermentation conditions about carbon,nitrogen,pH value and the culture time were analyzed by single factor experiment and orthogonal experiment.[Result] High PHA-producing strain 0206,which was isolated from activated sludge filter,was identified as Bacillus cohn through the physiological-biochemical identification.After optimization,fermentation conditions were:10 g/L glucose,10 g/L ammonium sulfate,2 g/L NaCl,medium pH 7.0,incubation time for 48 hours.[Conclusion] The study provides reference for fermentation of PHA.
关键词
PHA/生理生化鉴定/发酵条件优化Key words
PHA/Physiological-biochemical identification/Fermentation condition optimization引用本文复制引用
基金项目
济宁学院青年科研基金(2012QNKJ08)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据