火炭母乙醇提取物的抗氧化活性及其稳定性研究
Antioxidant Activity and Stability of Ethanol Extracts from Polygonum chinense Linn
王呈文 1余文森2
作者信息
- 1. 汕头职业技术学院自然科学系,广东汕头 515078
- 2. 华港集装箱分公司,广东汕头 515078
- 折叠
摘要
[目的]研究火炭母95％乙醇提取物的抗氧化活性及其稳定性.[方法]采用室温浸提法对火炭母中有效成分进行提取,考察其对1,1-二苯基-2-三硝基苯肼(DPPH·)、羟基自由基(OH·)、总抗氧化能力(ABTS法)、超氧阴离子(O2-·)的清除作用,并与Vc进行比较,以评价其抗氧化活性以及pH和温度对提取物稳定性的影响.[结果]火炭母95％乙醇提取物对DPPH·的清除率可达92.5％.提取物对DPPH·、OH·、ABTS+·、O2-·的半数抑制浓度(IC50)分别为32.8、90.8、110.8、277.1 μg/mL,清除能力均比Vc强,且提取物还具有较好的耐热性和耐弱酸弱碱性.[结论]火炭母95％乙醇提取物具有较强的抗氧化活性及良好的稳定性.
Abstract
[Objective] To research the antioxidant activity and stability of 95％ ethanol extracts from Polygonum chinense Linn.[Method] The effective components were extracted from P.chinense at room temperature by extraction method.Its scavenging effects on DPPH ·,OH ·,ABTS method and O2-· were researched.Vc was compared in order to evaluate the antioxidant activity.Effects of pH and temperature on the extracts stability were studied.[Result] Scavenging rate of 95％ ethanol extracts to DPPH · reached 92.5％.The IC50 of extracts to DPPH ·,OH ·,ABTS + · and O2-· were 32.8,90.8,110.8 and 277.1 μ,g/mL,respoctively.Their scavenging abilities were all stronger than Vc;and extracts had relatively better heat resistance,weak acid and alkali resistance.[Conclusion] 95％ ethanol extracts from P.chinense have relatively strong antioxidant activity and good stability.
关键词
火炭母/抗氧化/稳定性/乙醇提取物Key words
Polygonum chinense Linn./Antioxidant/Stability/Ethanol extracts引用本文复制引用
基金项目
汕头职业技术学院科研课题(SZK2014Y22)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据