摘要
[目的]调查猪嵴病毒在福建地区腹泻猪群中的流行和变异情况。[方法]根据 GenBank 中登陆的猪嵴病毒（porcine kobuvirus， PKoV）结构蛋白 VP1基因序列设计特异性引物，采用 RT －PCR 方法从某猪场采集腹泻小肠样品中扩增猪嵴病毒 VP1基因，将扩增后的目的片段克隆后进行序列测定。应用生物信息学软件，将获得的2株猪嵴病毒 VP1和 GenBank 的猪嵴病毒株 VP1基因序列进行对比分析。[结果]猪嵴病毒 CH ／FJNP／12L／2015与匈牙利株 K －30－HU ／2008／HUN（GQ249161）的核苷酸同源性最高，为88．1％，氨基酸同源性为95．3％。 CH ／FJNP／12W1／2015与越南株714441／CAOLANH －VH ／2012－2－21（KT266058）的核苷酸同源性最高，为88．2％，氨基酸同源性为96．1％，同源重组分析显示，2株毒株均无明显同源重组发生。[结论]频繁的畜禽国际贸易，以及如今便捷的现代化交通工具，人们生活提供方便的同时，也加速了猪嵴病毒毒性的传播。
Abstract
Objective] The current situation of the epidemic and genetic variation of the porcine Kobuvirus (PKV) in diarrhea pigs in Fujian province were investigated.[Method] The special primer was designed according to the sequence of PKV deposited in GenBank.The VP1 gene of PKV in intestinal samples collected from pig -raising farm was amplified with RT -PCR method, and then, the amplified target frag-ment was cloned and sequenced.The sequences of two PKV strains were analyzed and compared by means of bioinformatics software.[Re-sults] The results showed that there was high nucleotide homology between VP1 gene of CH /FJNP/12L /2015and K -30 -HU /2008 /HUN, which was 88.1%; amino acid homology, 95.3%.The homology in the nucleotide and amino acid of CH /FJNP/12W1 /2015 with 714441 /CAOLANH -VH /2012 -2 -21 was 88.2% and 96.1%, respectively.The two isolates had no obvious homologus recombination occurred with other strains.[Conclusion] speculated that the frequent international livestock trade, and is now a modern and convenient means of trans-port, in convenience to our lives, but also accelerate the spread of swine crest virus strains.[Conclusion] It is speculated that it resulted from the frequent international trade of livestock and poultry, and convenient modern transportation tools, which also accelerated the transmission of PKV.
国家科技期刊平台
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维普
万方数据