双分子荧光互补技术（BiFC）分析玉米 MAPK5与 bZIP72蛋白的相互作用
Interaction Between MAPK5 and bZIP72 of Maize Using Bimolecular Fluorescence Complementation (BiFC) Assay
曹建美1
作者信息
- 1. 中国科学院上海辰山植物研究中心，上海辰山植物园，上海松江201602
- 折叠
摘要
[目的]分析玉米 MAPK5与 bZIP72蛋白之间的相互作用。[方法]构建双分子荧光互补表达载体 pN －MAPK5和 pC －bZIP72，应用基因枪轰击洋葱表皮细胞瞬时表达，在激光共聚焦显微镜下观察 MAPK5与 bZIP72蛋白相互作用产生的荧光信号。[结果] Zm－MAPK5与 ZmbZIP72的 BiFC 融合载体同时轰击洋葱表皮细胞，细胞核中能发出黄色荧光。[结论] ZmMAPK5与 ZmbZIP72在植物细胞核内相互作用。
Abstract
Objective] To analyze the interaction between MAPK5 and bZIP72.[Method] Two BiFc plasmids (pN-MAPK5 and pC-bZIP72) were constructed, then transfected into onion epidermal cells via gene-gun approach.The interaction between MAPK5 and bZIP72 was examined by confocal laser scanning microscopy.[Result] Yellow fluorescence was observed in the onion epidermal cells transfected with ZmMAPK5 and ZmbZIP72.[Conclusion] This research indicates that ZmMAPK5 and ZmbZIP72 interacts with each other in vivo.
关键词
ZmMAPK5/ZmbZIP72/双分子荧光互补(BiFC)/蛋白互作Key words
ZmMAPK5/ZmbZIP72/Bimolecular fluorescence complementation (BiFC)/Protein interaction引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据