摘要
[目的]研究不同激素浓度对铁线莲诱导、增殖和生根的影响，建立铁线莲组培快繁技术。[方法]以铁线莲茎段为外植体，在MS培养基中加入不同浓度的6－BA、NAA和IBA，研究不同激素浓度对其腋芽诱导分化、诱导芽增殖和继代苗生根的影响。[结果]铁线莲腋芽诱导效果以MS＋6－BA 1．0 mg／L＋NAA 0．2 mg／L为最佳；增殖培养以MS＋6－BA 1．5 mg／L＋NAA 0．50 mg／L为最佳；继代苗生根培养以MS＋6－BA 1．5 mg／L＋IBA 2．0 mg／L最佳。[结论]建立了铁线莲组培快繁技术体系，为其规模化生产提供理论依据。
Abstract
Objective] The aim was to study effects of different hormone concentration on induction, proliferation and rooting of Clematis flori-da, to establish tissue culture rapid propagation technique of Clematis florida.[Method] With Clematis florida stem segments as explants, adding different concentrations of 6-BA, NAA, IBA growth hormone in MS culture medium , effects of different hormone concentration on induction dif-ferentiation, bud proliferation, and rooting culture of successive seedling were studied.[Result] Clematis florida axillary bud induction effects in MS +6-BA 1.0mg 0.2 mg/L way was the best;proliferation cultured in MS +6-BA 1.5 mg/L+NAA 0.5 mg /L treatment was the best;suc-cessive generation of rooting culture in process S3: MS+6-BA 1.5 mg/L+IBA 2.0 mg/L was the best.[Conclusion] The tissue culture rapid propagation technique system of Clematis florida is established, which can provide theoretical basis for large-scale production.
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