荨麻中3,4-二香草基四氢呋喃的提取及纯化
Extraction and Purification of 3,4-Two Vanillyl Tetrahydrofuran in Nettle
李静辉 1刘玲 1姚德坤 2黄运生1
作者信息
- 1. 大兴安岭科丽尔生物工程有限责任公司，黑龙江大兴安岭165012
- 2. 大兴安岭科丽尔生物工程有限责任公司，黑龙江大兴安岭165012; 大兴安岭林格贝寒带生物科技股份有限公司，黑龙江大兴安岭165012
- 折叠
摘要
[目的]研究荨麻中3,4-二香草基四氢呋喃的提取以及纯化,为进一步研究荨麻中3,4-二香草基四氢呋喃的作用机制奠定基础。[方法]采用醇水溶液回流提取,研究大孔吸附树脂吸附和分离狭叶荨麻提取液中3,4-二香草基四氢呋喃的方法和条件,并采用超滤膜纯化技术对其进行纯化。[结果]X-5大孔吸附树脂对3,4-二香草基四氢呋喃有较好的吸附能力；乙醇浓度为60%时解吸效果最好,解析液浓缩,超滤膜纯化重结晶后,荨麻中3,4-二香草基四氢呋喃纯度可达85.3%。[结论]采用X-5大孔吸附树脂以及超滤膜纯化技术可有效地提取荨麻中3,4-二香草基四氢呋喃,为今后荨麻中有效成分的研究与开发应用提供理论依据。
Abstract
Objective] The aim was to extract and purify 3,4-two vanillyl tetrahydrofuran in nettle, to lay a good basis for further research on the function mechanism. [ Method] Using alcohol aqueous solution for reflux extraction, the method and conditions for adsorption and sepa-ration of 3,4-two vanillyl tetrahydrofuran in nettle with macroporous adsorption resin were studied, ultrafiltration membrane purification tech-nology was adopted for purification. [Result] X-5 macroporous adsorption resin had better adsorption ability to 3,4-two vanillyl tetrahydro-furan;when ethanol concentration was 60%, the desorption effect was best, after the purification of the ultrafiltration membrane, purity of 3, 4-two vanillyl tetrahydrofuran in nettle could up to 85. 3%. [Conclusion] Using X-5 macroporous resin and ultrafiltration membrane purifi-cation technology can effectively extract 3,4- two vanillyl tetrahydrofuran in nettle, the study provides basis for research of 3,4- two vanillyl tetrahydrofuran in nettle.
关键词
荨麻/3,4二香草基四氢呋喃/提取纯化/大孔吸附树脂超滤膜偶联技术Key words
Nettle/3,4 -two vanillyl tetrahydrofuran/Extraction and purification/Macroporous adsorption resin ultrafiltration membrane coupling technique引用本文复制引用
基金项目
国家林业局948项目(2013-4-21)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据