芦笋“冠军”花药培养愈伤组织的诱导
The Callus Induction of Asparagus“Champion” Anther Culture
包艳存 1李书华 1李霞 1李芳1
作者信息
- 1. 山东省潍坊市农业科学院生物所，山东潍坊261041
- 折叠
摘要
[目的]研究芦笋“冠军”花药培养技术。[方法]以杂交一代品种“冠军”为试验材料,研究花药组培过程中影响愈伤组织诱导的因素及生长调节剂对愈伤分化成苗的影响。[结果]采用0.1%升汞灭菌5 min,再接种在添加NAA 0.8 mg/L+6-BA 2.0 mg/L的 MS固体培养基上先暗培养10 d,再光照培养,花药愈伤组织诱导率较高,达16.5%左右。[结论]建立了芦笋“冠军”花药培养技术体系,为开展芦笋全雄育种奠定基础。
Abstract
Objective] The aim was to study asparagus“champion” anther culture technique. [Method] Using the F1 hybrid asparagus variety“champion” as the test material, callus induction in tissue culture process and effects of growth regulator on callus differentiation were studied. [Result] The results showed that using 0. 1% mercuric chloride sterilization 5 min, inoculated on the MS solid medium by adding NAA0. 8 mg/L + 6-BA 2. 0 mg/L of darkness first 10 days, and then light culture, anther callus induction rate reached about 16. 5%. [Conclusion] The asparagus“champion” anther culture technical system is established, which will lay a foundation for carrying out asparagus all male breeding.
关键词
芦笋“冠军”/花药培养/愈伤诱导Key words
Asparagus officinalis“Champion”/Anther culture/Callus induction引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据