摘要
[目的]比较副猪嗜血杆菌国内流行血清型菌株感染后小鼠外周血淋巴细胞、血清细胞因子及免疫球蛋白水平的变化情况。[方法]将72只小鼠分为4组,分别腹腔注射副猪嗜血杆菌血清型4、5和12菌株,检测3 d、7 d和15 d各组外周血T、B淋巴细胞及血清细胞因子和Ig水平。[结果]副猪嗜血杆菌感染小鼠3 d,T、B淋巴细胞数量明显高于对照组,而随着感染时间的延长而逐渐下降。 IgG、IFN-γ和IL-10水平随感染时间的延长而逐渐增高,而IgA、IgM和IgE水平逐渐降低。感染3 d,Th1/Th2比值稍有下降,而Th1/Th2比值随着感染时间的延长而逐渐升高,明显高于对照组。不同血清型感染在各时间点之间T、B淋巴细胞、IgA、IgM、IgE、IFN-γ、IL-10和Th1/Th2差异均无统计学意义,而血清型4和5感染组小鼠血清IgG均明显高于血清型12组。[结论]除IgG以外,副猪嗜血杆菌国内流行血清型4、5和12菌株感染小鼠后外周血淋巴细胞、血清细胞因子及Ig水平无明显差异。
Abstract
Objective] To comparatively study on level of peripheral blood lymphocytes, cytokines, and immunoglobulins ( Ig) in mice infec-ted with the epidemic serotypes Haemophilus parasuis in China. [Method] 72 mice were subdivided into four groups, group serotypes 4, 5, 12, and PBS (control group), and were injected intraperitoneally with Haemophilus parasuis serotypes 4, 5, 12, and PBS. T lymphocytes and B lymphocytes, cytokines, and Ig were detected. [ Result] The number of T lymphocytes and B lymphocytes in mice on 3 days after infection with Haemophilus parasuis, and gradually decreased as increasing time. But gradually increased in the level of IgG, IFN-γ, and IL-10, and gradually decreased in the level of IgA, IgM, and IgE. The Th1/Th2 level was gradually increased as increasing time, and significantly higher than the control group. However, there were no significant differences between different serotypes Haemophilus parasuis in the level of T lym-phocytes and B lymphocytes, IgA, IgM, IgE, IFN-γ, IL-10, and Th1/Th2, but the level of IgG in group serotypes 4 and 5 were significantly higher than group serotypes 12. [Conclusion] There were no significant differences in the level of peripheral blood lymphocytes, cytokines, and Ig except IgG in mice infected with Haemophilus parasuis serotypes 4, 5, 12 in China.
国家科技期刊平台
NSTL
维普
万方数据