尾巨桉3个无性系继代增殖培养研究
Multiplication Subculture of Three Clones of Eucalyptus urophylla × E. grandis
石前 1李飞宇 2梁秀莉 1邓冬丽 1李丽芳 1兰俊1
作者信息
- 1. 广西壮族自治区国有东门林场，广西扶绥532108
- 2. 中种国际种子有限公司，北京100028
- 折叠
摘要
[目的]研究尾巨桉3个无性系继代增殖培养技术,为尾巨桉苗木快速繁殖提供理论依据。[方法]以尾巨桉3个无性系DH32-26、DH32-29、DH32-28为试验材料,筛选最佳的植物生长调节剂组合、凝固剂、暗培养天数和培养瓶,达到较高的继代增殖系数。[结果]3个无性系继代增殖培养时最适的植物生长调节剂组合为：DH32-26(6-BA 0.5 mg/L+NAA 0.2 mg/L)；DH32-28(6-BA 0.4 mg/L+NAA 0.2 mg/L)；DH32-29(6-BA 0.3 mg/L+NAA0.2 mg/L)；DH32-26的继代增殖系数在凝固剂为卡拉胶和琼脂的培养基中差异不大,DH32-28、DH32-29的继代增殖系数在以卡拉胶作为凝固剂的培养基中比在以琼脂作为凝固剂的培养基中有所提高,在生根培养过程中,以卡拉胶作为凝固剂的培养基远比用琼脂作为凝固剂的培养基诱导出根好；继代增殖初期DH32-26、DH32-28需用黑布进行全暗遮光5~10 d,而DH32-29不需全暗培养；用广口瓶继代增殖培养DH32-29可降低苗木生产成本,但DH32-26、DH32-28不适合用广口瓶进行继代增殖培养。[结论]要提高无性系继代增殖系数,需要选取最优的植物生长调节剂组合、凝固剂、暗培养天数和培养瓶。
Abstract
Objective] To research the multiplication subculture technology of three clones of Eucalyptus urophylla × E. grandis, and to pro-vide theoretical foundation for the rapid propagation of E. urophylla × E. grandis seedlings. [ Method] With three clones (DH32-26, DH32-29, DH32-28) as the test materials, the optimal combination of regulators was screened, as well as the coagulator, dark culture days and culture flask. Thus, multiplication coefficient was relatively high. [Result] The optimal combination of plant growth regulators for subculture of three clones were DH32-26(6-BA 0. 5 mg/L + NAA 0. 2 mg/L), DH32-28(6-BA 0. 4 mg/L + NAA 0. 2 mg/L) and DH32-29(6-BA 0. 3 mg/L +NAA 0. 2 mg/L). DH32-26 multiplication coefficient showed no significant differences in culture medium with carrageenan and agar as the coag-ulators. Subculture multiplication coefficients of DH32-28 and DH32-29 in culture medium with carrageenan as the coagulator was higher than those with agar as the coagulator. During the process of rooting, culture medium with carrageenan as the coagulator had far better rooting than that with agar as the coagulator. In the initial stage of DH32-26 and DH32-28 subculture, black cloth was used for shading for 5-10 d; while DH32-29 did not need all dark culture. DH32-29 subculture in wild-mouth bottle reduced the production cost of seedlings; but DH32-26 and DH32-28 were not suitable for the subculture in wild-mouth bottle. [Conclusion] To enhance the multiplication coefficient of clones, we should select the optimal regulators, coagulator, dark culture days and culture flask.
关键词
尾巨桉/无性系/继代增殖Key words
Eucalyptus urophylla × E. grandis/Clones/Multiplication引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据