HPLC－DAD－CAD法同时测定功能性饮料中牛磺酸和咖啡因含量
Simultaneous Determination of Taurine and Caffeine in Functional Drinks by HPLC-DAD-CAD
陈超 1宋玉梅 2梁慧 2朱桃玉 3陈智勇1
作者信息
- 1. 中国广州分析测试中心，广东广州510070
- 2. 广东省测试分析研究所，广东省分析测试技术公共实验室，广东广州510070
- 3. 赛默飞世尔科技 中国 有限公司，广东广州510030
- 折叠
摘要
[目的]无需衍生化样品，建立同时快速测定功能性饮料中牛磺酸和咖啡因含量的分析方法。[方法]采用高效液相色谱－二极管阵列检测器－电雾式检测器（HPLC－DAD－CAD）法，Poroshell 120 Hillic色谱柱（3．0 mm ×150 mm，2．7μm），乙腈和水不同比例梯度洗脱，流速0．5 mL／min，咖啡因的检测波长273 nm，建立标准曲线。功能性饮料经超声处理，用水稀释后，过滤膜，直接上机测定。[结果]试验表明，通过1次进样，同时测定了功能性饮料中牛磺酸和咖啡因的含量，且线性关系良好（r＝0．9984和0．9999），平均回收率为86．5％～104．4％，RSD值为0．4％～4．0％，牛磺酸和咖啡因的检出限分别为28．0 ng和3．5 ng。[结论]该方法简便、快速、灵敏，适用于功能性饮料中牛磺酸和咖啡因的含量测定。
Abstract
Objective] To establish the rapid method for simultaneous determination of taurine and caffeine in functional drinks without deri-vatization samples.[ Method] The high -performance liquid chromatography coupled with diode array and charged aerosol detector ( HPLC-DAD-CAD) was conducted on Poroshell 120 Hillic column (3.0 mm ×150 mm, 2.7 μm) with gradient elution.The mobile phase was aceto-nitrile and water, the flow rate was 0.5 mL/min, the detection wavelength of caffeine was 273 nm.A standard curve was established.Func-tional drinks were diluted with water after ultrasonic treatment.After the membrane filtration, they were tested directly on the machine.[ Re-sult] The taurine and caffeine in functional drinks were simultaneously detected by a single injection , showing good linear relationship ( r =0.998 4 and 0.999 9).The average recovery was 86.5% -104.4%, RSD was from 0.4% to 4.0%.The limits of detection of taurine and caffeine were 28.0 and 3.5 ng, respectively.[ Conclusion] The HPLC-DAD-CAD method is simple, rapid and sensitive, which can be ap-plied to test taurine and caffeine in functional drinks.
关键词
高效液相色谱-二极管阵列检测器-电雾式检测器联用法/牛磺酸/咖啡因/功能性饮料Key words
HPLC-DAD-CAD/Taurine/Caffeine/Content/Functional drinks引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据