基于血清药理学方法的头花蓼抗炎有效提取物筛选
Screeni ng of Effective Anti-inflammat oryE xtrac ts rf om Polygonum capitatumB ased on Serum Pharmacological Method
徐丹 1赵菲菲 2杨馨 2李靖 1徐国波 1廖尚高1
作者信息
- 1. 贵州医科大学药学院，贵州贵阳550004; 民族药与中药开发应用教育部工程研究中心，贵州贵阳550004
- 2. 贵州医科大学药学院，贵州贵阳550004; 贵州省药物制剂重点实验室，贵州贵阳550004
- 折叠
摘要
[目的]通过血清药理学方法，研究头花蓼不同提取物及热淋清颗粒含药血清对小鼠单核巨噬细胞（RAW264．7细胞）释放炎症因子的影响，筛选出头花蓼抗炎有效提取物。[方法]采用头花蓼血清药理学评价方法，10 ng／mL 脂多糖（ LPS）建立RAW264．7细胞炎症损伤模型，MTT法检测细胞存活率，Griess试剂法检测细胞上清液中NO的释放量，酶联免疫吸附法（ ELISA ）检测细胞上清液中TNF－α和IL－6的释放量，以考察头花蓼水提物、水提醇沉提取物、水提醇沉沉淀物及热淋清颗粒含药血清（ WEPS、PCWEPS、DPCWEPS、RLQS）对巨噬细胞释放炎症因子的影响。[结果]与模型组比较，WEPS、PCWEPS、DPCWEPS、RLQS均能显著抑制LPS刺激的RAW 264．7细胞释放炎症因子NO、TNF－α和IL－6，PCWEPS组抑制细胞释放NO、TNF－α和IL－6的作用最强。[结论]头花蓼水提醇沉提取物为头花蓼主要的抗炎有效提取物。
Abstract
Objective] To research the effects of different extracts from Polygonumc apitatum and drug-containing serum of Relinqing granules on releasing inflammatory factor of mouse monocyte-macrophage (RAW264 .7 cell) by serum pharmacological method, and to screen effective an-ti-nflammatory extracts from P.capitatum.[Method] Serum pharmacological evaluation method of P.capita tum was adopted.The inflammatory cell model was produced by LPS (10 ngm/L )-treated RAW264.7 cells.The cell viability was detected by MTT method.NO production was de-tected by the Griess assay,TNF -αand IL-6 were quantitated by the enzyme-linked immunosorbent assay (ELISA) in the cell culture supernatant to investigate the effect of drug-containing serum of Polygonum capitatum of its water extract (WEPS), protein-free water extract (PCWEPS), deposition of protein-free water extract (DPCWEPS) and Relinqing granules (RLQS) on inflammatory cytokines in macrophage cells.[Result] The drug-containing serums could significantly inhibit the release of NO , TNF-αand IL-6 in LPS-induced RAW264.7 cells compared with the model group (P<0.05, P<0.01), in which PCWEPS had the best effect on NO, TNF-αand IL-6 inhibitory.[Conclusion] Protein-free water extract of Polygonum capitatum is the main extract for anti-inflammatory.
关键词
头花蓼/热淋清颗粒/血清药理学/RAW/264.7细胞/炎症因子Key words
Polygonum capitatum/Relinqing granules/Serum pharmacology/RAW264.7 cells/Inflammatory cytokines引用本文复制引用
基金项目
国家自然科学基金(81160516)
国家自然科学基金(81560570)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据