摘要
[目的]分离纯化白蔹多糖，研究白蔹均一多糖对小鼠脾淋巴细胞增殖的影响。[方法]采用水提醇沉法提取白蔹多糖，通过三氯乙酸法脱除蛋白，用中空纤维超滤膜和DEAE Cellulose DE －52对白蔹多糖进行分离纯化，高效凝胶过滤色谱法鉴定纯度并测定分子量，PMP柱前衍生化测定单糖组成，采用MTT法测定均一多糖对ConA和LPS诱导的小鼠脾淋巴细胞增殖的影响。[结果] PAJM－A均一多糖分子量为1．29×105 u，由甘露糖（Man）、鼠李糖（Rha）、葡萄糖醛酸（GlcA）、半乳糖（Gal）、葡萄糖（Glc）、木糖（Xly）组成，其物质的量比为1．36∶1．87∶2．02∶1．58∶1．04，均一多糖对ConA诱导的T淋巴细胞和LPS诱导的B淋巴细胞有较显著的增殖作用。[结论]PA－JM－A是一种新的白蔹均一多糖，并与ConA、LPS 对T、B淋巴细胞的增殖有协同刺激作用。
Abstract
Objective] To separate and purify polysaccharides from Ampelopsis Japonica ( Thunb.) Makino, and to study its effects on prolif-eration function of mice spleen lymphocyte transformation.[Method] Hot water extracting and TCA method were employed to isolate the PA-JM.Hollow fiber ultrafiltration membrane and the DEAE Cellulose 52 were used to separate and purify polysaccharides, and the monosaccha-ride composition, and structure were preliminarily identified by IR and HPLC.Their effects on proliferation function of mice spleen lymphocyte transformation were determined by MTT.[Result] PAJM-A was 1.29 ×105 u and it was mainly composed of Man, Rha, GlcA, Glc, Gal and Xyl in the molar ratio of 1.36∶1.87∶2.02∶0.10∶1.58∶1.04.And the homogeneous polysaccharides were able to increase the proliferation of lymphocytes induced by ConA and LPS.[ Conclusion] PAJM-A is a novel homogeneous polysaccharide, PAJM-A can effectively increase lymphocyte proliferation and enhance the effect of ConA, LPS-induced T and B cell growth.
基金项目
佳木斯大学校级创新团队(CXTD－2013－05)
佳木斯大学青年创新人才培养计划(22 Zq201501)
佳木斯大学研究生科技创新项目(LZZ2015＿030)
国家科技期刊平台
NSTL
维普
万方数据