摘要
[目的]建立紫外可见分光光度法测定发酵蜈蚣粉中总黄酮类化合物含量的方法。[方法]通过醇水提取发酵蜈蚣粉中总黄酮类化合物，在510 nm下测定其吸光度并计算其含量。[结果]黄酮类化合物浓度在0．02～0．10 mg／mL时，其浓度与对应的吸光度呈良好的线性关系；在精密度试验中，发酵蜈蚣粉中总黄酮类化合物的平均吸光度为0．4205，RSD＝0．7407％；在重现性试验中，发酵蜈蚣粉中总黄酮类化合物的平均质量比为9．091 mg／g，RSD＝2．007％；在加样回收率试验中，其平均回收率为97．92％，RSD＝1．568％。[结论]采用紫外可见分光光度法对发酵蜈蚣粉中总黄酮类化合物含量进行测定，其精密度、重现性、回收率均良好，可用来测定发酵蜈蚣粉中总黄酮类化合物的含量。
Abstract
Objective] To establish a method that using UV-VIS spectrophotometry to determine total flavonoids content in fermented centi-pede powder.[Method] Through the aqueous alcohol extracting from fermented centipede powder, the absorbances at 510 nm of total fla-vonoids were determined and contents were calculated.[ Result] Concentration of flavonoids, within the scope of 0.02 to 0.10 mg/ml, its concentration and the corresponding absorbance showed good linear relationship;in the precision test of determination of total flavonoids in fer-mentation centipede powder, the average absorbance was 0.420 5, RSD =0.740 7%;in the reproducibility experiment, the average quality was 9.091 mg/g, RSD=2.007%.In the sample recovery rate test of determination, its average recovery was 97.92%, RSD =1.568%. [ Conclusion] By UV-VIS spectrophotometry for determination of total flavonoids content in fermented centipede powder , its precision, repeat-ability and recovery rate are good.So the method can be used to determine the flavonoids in fermented centipede powder.
基金项目
山东中医药大学大学生研究训练( SRT )计划资助项目(2015308)
国家科技期刊平台
NSTL
维普
万方数据