摘要
[目的]筛选沙棘品种深秋红茎尖组织培养基配方。[方法]选用不同培养基，以7月中旬至8月下旬采集的大田茎尖为外植体，对沙棘品种深秋红的组织培养技术进行研究。[结果]最适初代培养基为1／2MS＋6－BA 0．5 mg／L，最适愈伤组织诱导培养基为1／2MS＋6－BA 0．5 mg／L ＋IBA 0．5 mg／L，最适继代培养基为1／2MS＋6－BA 0．5 mg／L ＋IBA 0．2 mg／L，最适腋芽诱导培养基为1／2MS＋6－BA 0．5 mg／L。[结论]该研究建立了沙棘品种深秋红茎尖组织培养技术，为其产业化生产奠定基础。
Abstract
Objective] The aim was to screen out culture medium formula for stem tip of seabuckthorn variety Shenqiuhong.[Method] By using different culture mediums, with stem tip collected from middle Jul.to late Aug.as explants, tissue culture technology of seabuckthorn variety Shenqiuhong was studied.[Result] The optimal primary culture medium was 1/2MS+6-BA 0.5 mg/L, the optimal callus induction culture medium was 1/2MS +6-BA 0.5 mg/L +IBA 0.5 mg/L, the optimal subculture medium was 1/2MS +6-BA 0.5 mg/L +IBA 0.2 mg/L, the appropriate axillary bud induction medium was 1/2MS+6-BA 0.5 mg/L.[Conclusion] The cultivation technique for stem tip of seabuckthorn variety Shenqiuhong is established, which will lay a foundation for industrialized production.
基金项目
中央财政林业科技推广示范项目(ZYLYKJTG2015014)
国家科技期刊平台
NSTL
维普
万方数据