正交试验优化超声辅助豆腐柴总黄酮提取工艺
Optimizing the Ultrasound-assisted Extraction Techniques for Total Flavonoids from Premna microphylla Turcz by Orthogonal Test
黄宏轶 1黄玲艳 1张海燕 1李京雨 1孙翌 1彭景1
作者信息
- 1. 扬州大学食品科学与工程学院，江苏扬州225127
- 折叠
摘要
[目的]探索豆腐柴总黄酮的最佳提取工艺。[方法]在超声辅助和单因素试验基础上，采用三因素三水平正交试验优化得到豆腐柴总黄酮的最佳提取工艺。[结果]豆腐柴总黄酮最佳提取工艺为∶液料比为40∶1（mL／g），乙醇体积分数为70％，超声温度为70℃，提取时间为30 min。在此条件下，豆腐柴总黄酮提取率为83．1 mg／g。[结论]正交试验优化超声提取工艺能够显著提高豆腐柴总黄酮的提取率。
Abstract
Objective] To optimize the extraction techniques using for extracting total flavonoids from Premna microphylla Turcz.[Method] On the basis of ultrasound assisted and single factor test ,the optimal extraction technique was obtained through three factors and three levels orthogo-nal test.[ Result] The optimal extraction conditions were obtained as following:using 70% ethanol with a solid/liquid ratio of 40∶1 mL/g and extraction at 70℃for 30 min.Under the above conditions,83.1 mg/g flavonoids could be extracted from Premna microphylla.[Conclusion] The orthogonal test optimal ultrasound-assisted extraction technique can significantly improve the yield of total flavonoids from Premna microphylla.
关键词
豆腐柴/总黄酮/正交试验/工艺优化Key words
Premna microphylla Turcz/Total flavonoids/Orthogonal test/Technique optimization引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据