摘要
[目的]探讨雪白弯颈霉（Tolypocladium inflatum）产环孢菌素的最适发酵条件。[方法]采用正交试验，研究不同培养时间、培养基种类、装瓶量、菌种接种量（以菌片计）对雪白弯颈霉产环孢菌素的影响。[结果]在培养时间为4 d，发酵培养基为马铃薯200．00 g、甘露醇40．00 g、蛋白胨6．00 g，装瓶量为100 mL，菌片投放为9片时雪白弯颈霉产环孢菌素的发酵条件为最优。[结论]试验结果为提高雪白弯颈霉产环孢菌的产量提供了理论依据。
Abstract
Objective] To discuss the optimum fermentation conditions for the production of cyclosporin from Tolypocladium inflatum.[ Meth-od] Through an orthogonal experiment, the effects of culture time, culture medium, bottling capacity, and inoculation amount ( in slice) of T.inflatum on the production of cyclosporin were analyzed.[ Result] The optimum fermentation conditions for the production of cyclosporin from T.inflatum were shown as follows:the culture time was 4 d;the fermentation culture medium was composed of potato 200.00 g, manni-tol 40.00 g, and peptone 6.00 g;bottling capacity was 100 mL;inoculation amount was 9 slices.[ Conclusion] The study can provide theo-retical foundation for the improvement of yield of cyclosporin produced from T.inflatum.
基金项目
大连民族大学人才引进科研项目启动基金(0701－110048)
中央高校自主科研基金(DC201502070402)
大学生创新创业训练项目(XA201612317)
大学生创新创业训练项目(XA201612315)
国家科技期刊平台
NSTL
维普
万方数据