超滤膜分离牡蛎多糖工艺研究
Optimization Process for Separation of Polysaccharides from Crassostrea gigas Using Ultrafiltration Membrane
郑亚旭 1石璇 1李欣遥 1佟长青 1李伟1
作者信息
- 1. 大连海洋大学食品科学与工程学院，辽宁大连116023
- 折叠
摘要
[目的]获得制备牡蛎多糖的最佳分离工艺。[方法]利用超滤膜对牡蛎多糖进行分离，采用单因素及正交试验考察牡蛎多糖通过不同截留分子量超滤膜的分子量分布并优化超滤膜分离工艺。[结果]试验表明，选择截留分子量10 kD的超滤膜分离牡蛎多糖，膜通量较好，截留液中牡蛎多糖的分子量为1．2×103 kD，透过液中的分子量为4．4 kD。正交试验表明，影响膜通量的主要因素是料液温度，其次是膜分离操作压力，pH影响较弱。综合考虑生产成本及膜的使用寿命等多方因素，超滤膜分离最佳工艺为料液温度30℃、压力0．04 MPa、pH 7．0。[结论]研究可为工业化生产牡蛎多糖提供参考依据。
Abstract
Objective] To optimize the methods of separating polysaccharides from Crassostrea gigas (CGPS).[Methods] Ultrafiltration membrane was used, single factor and orthogonal experiment were carried, and the molecular weight distribution and process of CGPS were studied.[Results] Results indicated that the flux of ultrafiltration membrane with molecular weight cut off of 10 kD was was better.The CGPS cut off by the membrane hasmolecular weight with 1.2 ×103 kD, and the permeate liquor has molecular weight of 4.4 kD.The orthogonal tests showed the main influencing factors are temperature of solution and operating pressure, while pH had less influence.In overall consideration of the life of membrane and production costs, the optimum technology of ultrafiltration are temperature 30 ℃, operating pressure 0.04 MPa and pH 7.0.[Conclusion] This study is expected to provide certain reference for extracting.
关键词
超滤膜/牡蛎多糖/最佳工艺/分子量分布Key words
Ultrafiltration membrane/Crassostrea gigas polysaccharides ( CGPS)/Optimum technology/Molecular weight distribution引用本文复制引用
基金项目
海洋局公益性行业科研专项项目(201405017－03)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据