不同基础培养基对漏斗形马勃菌丝体生长的影响
Effects of Different Basic Media on the Mycelium Growth of Lycoperdon excipuliforme
王君 1田强 1游朗 1李玉延 1宋力雅 1马林2
作者信息
- 1. 西南科技大学生命科学与工程学院，四川绵阳621010
- 2. 西南科技大学生命科学与工程学院，四川绵阳621010; 四川省生物质资源利用与改性工程技术研究中心，四川绵阳621010
- 折叠
摘要
[目的]研究漏斗形马勃在7种不同基础培养基上的生长情况。[方法]采用7种基础培养基进行试验，通过液体和固体培养，以菌丝体生物量及菌落直径为指标，确定漏斗形马勃的最佳基础培养基。[结果]最适合漏斗形马勃生长的基础培养基配方为玉米渣4％、蛋白胨0．3％、蔗糖2％、KH2 PO41％、MgSO40．05％。在28℃和摇床转速150 r／min 条件下，液体培养5 d 后的菌丝体干重为16．56 g／L。在28℃固体培养7 d，菌丝体鲜重和干重分别为16．47和2．27 g／L，菌落直径为4．49 cm。[结论]该研究为漏斗形马勃培养条件的进一步优化奠定了基础。
Abstract
Objective] To study the mycelium growth of Lycoperdon excipuliforme in seven basic media.[Method] According to medium biomass and colony diameter as growth parameters,the optimal basic medium for the mycelium growth of L.excipuliforme was selected from seven liquid and solid media with different constituents.[Result] The optimal basic medium for the mycelium growth of L.excipuliforme was the IV medium composed of maize (4%),peptone (0.3%),sucrose (2%),KH2PO4(1%) and MgSO4(0.05%).After the mycelia were cultured in the liquid medium at the rotational speed of 150 r/min at 28 ℃for 5 d,their dry weight in the IV medium was 16.56 g/L.When the mycelia were cultured in solid medium at 28 ℃for 7 d,their fresh weight and dry weight in the IV medium were 16.47 and 2.27 g/L respectively,and the colony diam-eter was 4.49 cm.[ Conclusion] The study lays the foundation for the further optimization of culture conditions of L.excipuliforme.
关键词
漏斗形马勃/固体培养/液体培养/培养基/菌丝体/生长Key words
Lycoperdon excipuliforme/Solid culture/Liquid culture/Culture medium/Mycelium/Growth引用本文复制引用
基金项目
国家大学生创新创业训练计划(201410619002)
西南科技大学大学生创新基金(CX15－040)
西南科技大学生命科学与工程学院大学生创新实践项目(2014011)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据