摘要
[目的]优化枯草芽孢杆菌CK15的发酵条件，提高其芽孢产量。[方法]通过单因素试验优化碳源种类及浓度、氮源种类及浓度、无机盐种类、装液量、摇床转速、初始pH、温度、接种量，采用Plackett－Burman试验筛选出培养基中的显著因素，再利用Box－Behnken试验确定3个因素的最佳浓度。[结果]在玉米粉10．7 g／L、豆粕粉24．4 g／L、CaCO37．4 g／L、NaCl 5．0 g／L、MnSO40．4 g／L、KH2 PO41．0 g／L、装液量50 mL／250 mL、转速为200 r／min、初始pH 7．2、温度30℃、接种量2．0％条件下，芽孢产量达到7．4×109 cfu／mL，比优化前提高了80．49％。[结论]响应面法有效提高了枯草芽孢杆菌CK15的芽孢产量。
Abstract
Objective] To optimize the fermentation condition of Bacillus subtilis CK15 and to enhance the Spore Production yield.[ Method] Single factor test was used to optimize the type and concentration of carbon source, type and concentration of nitrogen source, the type of inor-ganic salt, loaded liquid volume, shaker speed, initial pH, temperature and inoculum size.The optimal concentrations of three factors were determined by Plackett-Burman design; and the optimal concentrations of three factors were obtained by Box-Behnken test.[ Result] The spore production was 7.4 ×109 cfu/mL under the conditions of 10.7 g/L corn flour, 24.4 g/L soybean meal powder, 7.4 g/L CaCO3 , 5 g/L NaCl, 0.4 g/L MnSO4 , 1.0 g/L KH2 PO4 , 50 mL/250 mL loaded liquid volume, 200 r/min rotation speed, initial pH 7.2, 30 ℃ tempera-ture, and 2 %inoculation amount.Thus, the spore production increased by 80.49%than before.[Conclusion] The response surface method effectively improves the spore production of Bacillus subtilis CK15.
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