应用分子标记辅助选育抗褐飞虱水稻两系不育系
Breeding TGMS Lines with Resistanc e to Brown Planthopper by Marker-Assisted Selection
徐晓明 1程攀 1陈龙 1曲姗姗 1阴云伙 1田发春 1彭炳生 1吴帅 1李土明 1周卫营1
作者信息
- 1. 江西红一种业科技股份有限公司，江西南昌330046
- 折叠
摘要
[目的]选育抗褐飞虱水稻两系不育系。[方法]以B5为供体材料，采用分子标记辅助选择，将B5所含的Bph14和Bph15基因导入两系不育系1892S，获得符合两用核不育系标准的株系S136、S179、S192。[结果]分子标记检测结果表明，3个株系分别携带Bph14＋Bph15；人工接虫鉴定结果显示，3个两系不育系都高抗褐飞虱。[结论]该研究为进一步选育抗褐飞虱杂交稻组合提供了亲本材料。
Abstract
Objective] The TGMS Line with the resistance to brown planthopper was bred.[Method] The gene: Bph14 and Bph15, con-tained in rice material-B5 was transferred into the TGMS Line 1892S by means of marker-assisted selection ( MAS) and three TGMS lines (S136, S179 and S192) were successfully developed.[Result] The result of molecular test and evaluation showed that three lines were with the Bph14 and Bph15 and the identification in artificial inoculation of insect indicated that the resistance of three lines to BPH was high.[ Con-clusion] The rice material was provided for the breeding of the hybrid rice combination with the resistance to BPH.
关键词
水稻/两系不育系/抗褐飞虱/分子标记辅助选择/选育Key words
Rice/TGMS line/Brown planthopper/MAS/Breeding引用本文复制引用
基金项目
江西省科技型中小企业技术创新基金(赣科发计字201526号)
国家农业科技成果转化项目(赣科发农字2014150号)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据