长双歧杆菌NCC2705分泌蛋白的全基因组预测和功能分析
Genome-wide and Function Analysis of the Secretory Proteins of Bifidobacterium longum NCC2705
朱德全 1王茗悦 2栗金柳 2孟祥晨 3程广东 2岳丽红 2王长平 2薛勇2
作者信息
- 1. 佳木斯大学生命科学学院，黑龙江佳木斯154007; 东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨150030
- 2. 佳木斯大学生命科学学院，黑龙江佳木斯154007
- 3. 东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨150030
- 折叠
摘要
以长双歧杆菌NCC2705基因组序列为研究对象，使用SignalP 4．0、LipoP、TMHMM 2．0软件分析该基因组中的分泌蛋白及其信号肽的类型，同时采用COG （ Cluster of Orthologous Groups of proteins）功能数据库对预测的分泌蛋白进行功能注释和聚类分析。结果表明，长双岐杆菌NCC2705中共有37个Sec途径分泌蛋白，其中Sec途径分泌蛋白包括27个被I型（ SPaseⅠ）信号肽酶和10个Ⅱ型信号肽酶（ SpaseII）识别的蛋白，Sec 途径分泌蛋白信号肽长度最多的有52个氨基酸，最少的有10个氨基酸。分泌蛋白的功能分析表明，该菌株分泌蛋白中含有大部分的假定蛋白，主要参与氨基酸代谢与转运、碳水化合物代谢转运，无机盐离子代谢转运，细胞壁和细胞膜生物合成的功能有关。
Abstract
With the genome proteins sequences of Bifidobacterium longum FNCC2705 as study objects,SignalP 4.0,LipoP,TMHMM 2.0 software were used to analyze the secretory proteins and types of signal peptide.Function of secretory proteins was also analyzed by COG ( Cluster of Or-thologous Groups of proteins) database.Results showed a total of 37 secretion proteins through Sec pathway which included 27 secreted proteins recognized by SPaseⅠand 10 secreted proteins recognized by SPaseⅡ.Maximum length of their signal peptide is 52 aa and minimum length is 10 aa respectively.The functions of Bifidobacterium secretory protein showed there were many hypothetical proteins.Secretory protein with annotation of COG functions were mainly involved in amino acid transport and metabolism,carbohydrate transport and metabolism,inorganic ion transport and metabolism,etc..The functions of these secretory proteins can help bifidobacterium adapt to its host environment.
关键词
长双歧杆菌/基因组/分泌蛋白/分泌途径/信号肽Key words
Bifidobacterium longum/Genome/Secreted proteins/Secretion pathways/Signal peptide引用本文复制引用
基金项目
佳木斯大学科研项目(S2011－049)
黑龙江省卫生计生委科研项目(2016－325)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据