利用实时荧光P CR技术快速检测玉米籽粒中转基因成分
Quickly Detection of Transgenic Ingredients in Corn Grain by Real-time Fluorescent PCR Technology
王颖 1任志莹 1陈芳芳1
作者信息
- 1. 农业部农产品质量监督检验测试中心 沈阳，辽宁沈阳110161
- 折叠
摘要
[目的]利用TaqMan特异性荧光标记法快速检测玉米籽粒中转基因成分。[方法]外源基因选取具有一定代表性的CaMV35S启动子、NOS终止子、BAR、CryIA（b）基因，数据结果由实时荧光定量PCR反应的Ct值判定。[结果]用该方法检测玉米籽粒中未含有转基因成分，可作为结果判定。[结论]该方法在样品量大时极具优势，高效，准确，对环境污染较小。
Abstract
Objective] To rapidly detect the transgenic ingredients in corn grain by TaqMan specificity fluorescent tags.[Method] CaMV35S promoters and NOS terminator, BAR, CryIA(b) gene with certain representativeness were selected as exogenous genes.Data results were judged by Ct value of real-time fluorescent quantitative PCR reaction.[Result] corn grain without transgenic ingredients detected by this method could be used as the judgement result.[Conclusion] This method is of most advantage under large sample amount.It is efficient and accurate, and has less pollution to the environment.
关键词
转基因玉米/实时荧光定量PCR/特异性/外源基因Key words
Transgenic corn/Real-time fluorescent quantitative PCR/Specificity/Exogenous gene引用本文复制引用
出版年
2016
国家科技期刊平台
NSTL
万方数据