玉米磷酸烯醇式丙酮酸羧化酶（PEPC）基因的克隆及序列分析
Cloning and Bioinformatics Analysis of Phosphoenolpyruvate Carboxylase(PEPC) in Maize
王重 1樊哲儒 1张跃强 1李剑峰 1高新1
作者信息
- 1. 新疆农业科学院核技术生物技术研究所，新疆乌鲁木齐830091; 农业部荒漠绿洲作物生理生态与耕作重点实验室，新疆乌鲁木齐830091
- 折叠
摘要
[目的]获得玉米的PEPC基因，并对其生物信息学进行分析。[方法]使用RT－PCR方法由玉米中获得PEPC全长cDNA，构建克隆载体后进行测序，并对其序列进行生物信息学分析。[结果]获得的玉米PEPC基因CDS全长2913 bp，编码的多肽链包含970个氨基酸，为疏水性氨基酸，由α－螺旋（61．44％）、无规则卷曲（34．43％）和延伸链（4．12％）组成，定位于细胞质，不存在跨膜结构域，其175～970位氨基酸组成了磷酸烯醇式丙酮酸羧化酶的功能结构域，在173～184、597～609位具有2个磷酸烯醇式丙酮酸羧化酶活性位点。[结论]该研究克隆了玉米C4型丙酮酸磷酸双激酶（PPDK）基因，它所编码的氨基酸序列具备PPDK蛋白的保守序列和催化活性中心区域。
Abstract
Objective] To clone the phosphoenolpyruvate carboxylase(PEPC) gene obtained from Zea mays and analyze it by bioinformatics. [ Method] Primarily, the cDNA clone was constructed by RT-PCR amplified with the gene specific primers, then positive clones were se-quenced and analyzed by bioinformatics.[ Result] The electrophoresis showed that the coding sequence( CDS) of PEPC gene in Zea mays was 2 913 bp and the polypeptide chains coded by PEPC included 926 amino acids, which were hydrophobic amino acids consists of-helix (61.44%), random coil (34.43%) and extended strand (4.12%), with located in the cytoplasm.There was no transmembrane domain. The 175-970 amino acid composition the functional domains of phosphoenolpyruvate carboxylase, in 173-184;597-609 bits had two phos-phoenolpyruvate carboxylase active site with pyruvate orthophosphate dikinase basing on amino acids sequences comparison.[ Conclusion] The PEPC gene in Zea mays has been obtained and the amino acids sequence coded by the gene was conserved and contained active catalyst central site of PEPC.
关键词
C4/植物/光合作用效率/PEPC/生物信息学分析Key words
C4 plant/Photosynthetic efficiency/PEPC/Bioinformatics analysis引用本文复制引用
基金项目
新疆农业科学院青年基金(xjnkq－2013025)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据