摘要
[目的]优化巨大芽孢杆菌BM05的发酵条件，提高其发酵活菌数。[方法]通过单因素试验筛选最佳碳、氮源，通过正交试验筛选最佳C／N比，并研究pH、发酵温度、接种量和转速对发酵的影响。[结果]碳源选用玉米淀粉和葡萄糖，氮源选择牛肉粉，初始pH 7．0，发酵温度35℃，接种量为4％，转速220 r／min为最佳发酵条件。通过发酵条件优化，发酵菌数达4．73×109 cfu／mL，比原始配方提高了1.56倍。[结论]优化后的发酵条件大大提高了巨大芽孢杆菌BM05的发酵活菌数。
Abstract
Objective] To optimize the fermentation condition of Bacillus megaterium BM05, and to enhance the fermented bacterial count. [Method] The optimal carbon source and nitrogen source were selected by single factor test, while the optimal carbon-nitrogen ratio was se-lected by orthogonal test.And the pH, fermentation temperature, inoculation quantity and rotation speed were also studied.[ Result] Corn starch and glucose were the optimal carbon source and beef powder was the optimal nitrogen source.The optimal fermentation conditions were initial pH 7.0, 35 ℃ temperature, 4% inoculation quantity and 220 r/min rotation speed.Bacillus megaterium BM05 reached 4.73 ×109 cfu/mL after the optimization of fermentation condition, which was 1.56 times higher than the control.[Conclusion] The optimized fermenta-tion condition greatly enhances the fermented bacterial count of Bacillus megaterium BM05.
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