摘要
[目的]考察加压协同萃取艾叶中挥发油、总黄酮和鞣酸的最佳工艺条件。[方法]采用加压辅助溶剂萃取，从艾叶中同步提取挥发油、总黄酮和鞣酸，通过单因素试验和正交试验优化工艺条件。[结果]加压对艾叶化学成分的提取率影响显著；确定加压溶剂萃取艾叶中挥发油、总黄酮和鞣酸的最佳工艺条件为溶剂浓度70％、固液比1∶30（g／mL）、萃取压力0．9 MPa、萃取时间40 min、萃取温度90℃，在此试验条件下，挥发油、总黄酮和鞣酸的提取率分别为0．852％、4．660％、6．790％。[结论]与提取艾叶化学成分的其他方法相比，加压溶剂萃取可实现艾叶中挥发油、总黄酮和鞣酸的同步提取。该工艺条件稳定，提取时间短，溶剂利用率高，艾叶中3种成分的提取效果较理想。
Abstract
Objective] To investigate the optimal technological condition for pressurized synchronously extraction of volatile oil, total flavonoids and tannic acid from Artemisia argyi.[Method] By using pressurized extraction, the volatile oil, total flavonoids and tannic acid were extracted synchronously from A.argyi.The extraction technology was optimized by single factor test and orthogonal test.[ Result] Pressurization had sig-nificant effects on the extraction rate of chemical composition in A.argyi.The optimal extraction technology for volatile oil, total flavonoids and tannic acid from Artemisia argyi was as follows:70% ethanol concentration, 1∶30 ( g/mL) solid-liquid ratio, 40 min extraction time, 90 ℃ ex-traction temperature, and 1.0 MPa extraction pressure.Under these conditions, the extraction rate of volatile oil, total flavonoids and tannic acid were 0.852%, 4.66%and 6.79%, respectively.[Conclusion] Compared with other extraction methods of chemical components from A.argyi, pressurized extraction can realize simultaneously extraction of volatile oil, total flavonoids and tannic acid from A.argyi.This technological con-dition is stable, the extraction time is short and solvent utilization is high.Extraction effects of the three components in A.argyi leaves were rela-tively ideal.
基金项目
永州市2015年度第二批指导性科技计划项目(永科发201510号)
万方数据