Objective]To discuss the distribution of polysaccharides in mice after oral injection with Dendrobium huoshanense polysaccharide. [Method]Conjugating the polysaccharides to bovine serum albumin as an antigen, monoclonal antibodies were obtained and used as primary anti-body to conduct immuno histochemistry experiments.[ Result] It was confirmed that CDAP activation method can effectively couple polysaccha-rides and proteins, the obtained conjugates can induce the immune response of mice to produce the required monoclonal antibody.Distribution of polysaccharides in mouse tissues was obtained by using monoclonal antibody with high activity and specificity.[Conclusion] The method can pro-vide scientific basis for distribution of Dendrobium huoshanense polysaccharide in mice.
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