摘要
[目的]采用高速逆流色谱法（HSCCC）分离纯化雪白睡莲花中的酚类成分。[方法] HSCCC分离过程分为两步，分别采用乙酸乙酯∶甲醇∶水（4∶1∶5）和乙酸乙酯∶正丁醇∶水（4∶1∶5）两个体系，固定相均采用上相，流动相均采用下相，流速为2．0 mL／min，主机转速设定为850 r／min，紫外检测波长为254 nm。高效液相色谱（HPLC）检测纯度。[结果]第一步从200 mg 的睡莲花提取物中分离得到异槲皮苷（I，41．4 mg）、紫云英苷（II，40 mg）和一混合物85 mg；第二步从混合物中分离纯化得到isostrictiniin（III，50．1 mg）和烟花苷（IV，26．3 mg），上述4种物质的纯度分别为73．53％、92．42％、82．36％和85．65％。[结论] HSCCC可快速的分离纯化雪白睡莲花中的酚类成分。
Abstract
Objective] To isolate and purify phenolic constituents from Nymphaea candida Presl by high-speed counter-current chromatogra-phy ( HSCCC) .[ Methods] HSCCC was employed to separate phenolic compounds from N.candida with two steps.Two solvent systems were ethyl acetate ∶methanol ∶water (4∶1∶5) and ethyl acetate ∶n-butanol ∶water (4∶1∶5).HSCCC was executed under the conditions of 2.0 mL/min flow rate, 850 r/min rotation speed and 254 nm detection wavelength.The purities of compounds were determined by HPLC. [Result] In the first separation step, isoquercitrin (I, 41.4 mg), astragalin (II, 40 mg) and mixtures of III and IV (85 mg) were extracted from 200 mg sample.In the second step, mixtures of III and IV were obtained:isostrictiniin (III, 50.1 mg) and nicotiflorin(IV, 26.3 mg). The purities of these compounds were determined as 73.53%, 92.42%, 82.36% and 85.65%by HPLC.[Conclusion] Phenolic compounds from N.candida can be quickly isolated and purified by HSCCC.
基金项目
新疆维吾尔自治区科技支撑计划项目(20133318)
万方数据