摘要
[目的]筛选一种能够得到高质量桑树基因组DNA的方法。[方法]采用天根试剂盒法（Plant Genomic DNA Kit 离心柱型），并稍加改进，从11个桑种的新鲜嫩叶中提取DNA。[结果]该方法简单快速，不需要复杂仪器，且能有效地除去桑叶中的多糖、多酚、蛋白质等杂质，得到的DNA能够满足RAD－Seq高通量的测序要求。[结论]建立了一种用于桑RAD－Seq高通量测序的基因组DNA提取方法。
Abstract
Objective] The aim was to screen out a method to obtain high quality DNA from mulberry genome.[Method] By using improved Plant Genomic DNA Kit centrifugal column method, DNA was extracted from 11 species of fresh mulberry leaves.[Result] The method is sim-ple, rapid, and can effectively remove polysaccharides, polyphenols, protein and other impurities in the mulberry leaves, the DNA is able to meet the RAD-Seq high-throughput sequencing requirements.[Conclusion] A DNA extraction method for Morus RAD-Seq sequencing was estab-lished.
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