摘要
[目的]研究牛大力组织培养与快速繁殖技术。[方法]以牛大力种子为外植体，采用以芽繁芽途径，比较3种不同种源牛大力种子无菌发芽率、继代增殖、生根培养的表现差异。[结果]以MS＋6－BA 0．4 mg／L＋NAA 0．2 mg／L为诱导培养基，种子无菌发芽率分别为98．23％、92．13％和95．01％；以改良MS＋6－BA 0．6 mg／L ＋IBA 0．3 mg／L＋KT 0．2 mg／L为继代增殖培养基，添加半胱氨酸8 mg／L， FeSO4·7H2 O加至MS用量的1．2倍，增殖系数分别达5．57、3．20、5．30；以1／2MS＋NAA 1．0 mg／L＋IBA 1．5 mg／L、1／2MS＋NAA 1．0 mg／L＋IBA 3．0 mg／L、1／2MS＋NAA 1．5 mg／L＋IBA 1．0 mg／L为生根培养基，生根率分别达78．00％、67．33％、70．33％；生根苗移栽在V黄心土∶V细河沙∶V泥炭土＝8∶1∶1基质中培育60 d后，成活率分别达94．00％、85．43％和89．97％。采用控根容器培育大苗，可实现育苗与种植同步。[结论]该研究为牛大力种苗快速繁殖及产业化生产提供科学依据。
Abstract
Objective] The aim was to research the tissue culture and rapid propagation of Millettia speciosa.[ Method] Using M.speciosa seeds as explant, the seeds germination rate, the proliferation coefficient, the rooting rate of M.speciosa from three provenances were compared.[Re-sult] The results showed that the seeds germination rate was 98.23%, 92.13% and 95.01% respectively under sterile condition in medium of MS+6-BA 0.4 mg/L+NAA 0.2 mg/L, the proliferation coefficient was 5.57, 3.20 and 5.30 respectively in medium of modified MS+6-BA 0.6 mg/L +IBA 0.3 mg/L+KT 0.2 mg/L, added with cysteine 8 mg/L and 1.2 times of FeSO4 · 7H2 O to MS, the rooting rate was 78.00%, 67.33% and 70.33% respectively in medium of 1/2MS+NAA 1.0 mg/L+IBA 1.5 mg/L,1/2MS+NAA 1.0 mg/L+IBA 3.0 mg/L and 1/2MS+NAA 1.5 mg/L+IBA 1.0 mg/L, and the transplanting survival rate was 94.00%, 85.43%and 89.97%respectively on yellow sub-soil, fine sand and peat soil at the rate of 8∶1∶1.The root controlling container seedlings cultivation, breeding and planting can achieve synchro-niaztion.[Conclusion] The research can provide the scientific basis for seedling rapid propagation and industrial production of M.speciosa.
基金项目
钦州市科学研究与技术开发计划项目(20143002)
万方数据